TY - JOUR
T1 - Posthumous sperm retrieval
T2 - Analysis of time interval to harvest sperm
AU - Shefi, Shai
AU - Raviv, Gil
AU - Eisenberg, Michael L.
AU - Weissenberg, Ruth
AU - Jalalian, Liza
AU - Levron, Jacob
AU - Band, Gili
AU - Turek, Paul J.
AU - Madgar, Igael
N1 - Funding Information:
*Recipient of fellowship from The American Physicians Fellowship for Medicine in Israel.
PY - 2006/11
Y1 - 2006/11
N2 - Background:Current recommendations regarding posthumous sperm retrieval (PSR) are based on a small number of cases. Our purpose was to determine the time interval from death to a successful procedure. Methods: Sevent een consecutive PSR procedures in 14 deceased and 3 neurologically brain-dead patients at two male infertility centres [Sheba Medical Center (SMC), Tel-Hashomer, Israel and University of California San Francisco (UCSF), San Francisco, CA, USA] were analysed. Main outcome measures were retrieval of vital sperm, pregnancies and births. Results: PSR methods included resection of testis and epididymi s (n = 8), en-block excision of testis, epididymis and proximal vas deferens with vasal irrigation (n = 6), electroejaculation (EEJ) (n = 2) and epididymectomy (n = 1). PSR was performed 7.5-36 h after death. Sperm was retrieved in all cases and was motile in 14 cases. In two cases, testicular and epididymal tissues were cryopreserved without sperm evaluation, and in one case, no motility was detected. IVF and ICSI were performed in two cases in which sperm had been retrieved 30 h after death, and both resulted in pregnancies and live births. Conclusions: V iable sperm is obtainable with PSR well after the currently recommended 24-h time interval. PSR should be considered up to 36 h after death, following appropriate evaluation. No correlation was found between cause of death and chance for successful sperm retrieval.
AB - Background:Current recommendations regarding posthumous sperm retrieval (PSR) are based on a small number of cases. Our purpose was to determine the time interval from death to a successful procedure. Methods: Sevent een consecutive PSR procedures in 14 deceased and 3 neurologically brain-dead patients at two male infertility centres [Sheba Medical Center (SMC), Tel-Hashomer, Israel and University of California San Francisco (UCSF), San Francisco, CA, USA] were analysed. Main outcome measures were retrieval of vital sperm, pregnancies and births. Results: PSR methods included resection of testis and epididymi s (n = 8), en-block excision of testis, epididymis and proximal vas deferens with vasal irrigation (n = 6), electroejaculation (EEJ) (n = 2) and epididymectomy (n = 1). PSR was performed 7.5-36 h after death. Sperm was retrieved in all cases and was motile in 14 cases. In two cases, testicular and epididymal tissues were cryopreserved without sperm evaluation, and in one case, no motility was detected. IVF and ICSI were performed in two cases in which sperm had been retrieved 30 h after death, and both resulted in pregnancies and live births. Conclusions: V iable sperm is obtainable with PSR well after the currently recommended 24-h time interval. PSR should be considered up to 36 h after death, following appropriate evaluation. No correlation was found between cause of death and chance for successful sperm retrieval.
KW - Assisted reproduction
KW - Male infertility
KW - Posthumous
KW - Sperm retrieval
UR - http://www.scopus.com/inward/record.url?scp=33749986053&partnerID=8YFLogxK
U2 - 10.1093/humrep/del232
DO - 10.1093/humrep/del232
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C2 - 16959804
AN - SCOPUS:33749986053
SN - 0268-1161
VL - 21
SP - 2890
EP - 2893
JO - Human Reproduction
JF - Human Reproduction
IS - 11
ER -