@article{be242fd44b9843e3a5a5ab5ff276e147,
title = "Possible role of factor XIII subunit A in Fcγ and complement receptor-mediated phagocytosis",
abstract = "Besides its traditional role in hemostasis, factor XIII subunit A (FXIII-A) is supposed to function as a cellular transglutaminase and to be involved in certain intracellular processes, including cytoskeletal remodeling. To investigate its intracellular role, the aim of the present study was to follow changes in FXIII-A production in combination with the receptor-mediated phagocytic activities of monocytes/macrophages and to examine the phagocytic functions of monocytes in patients with FXIII-A deficiency. Human blood monocytes were isolated from the buffy coats of healthy volunteers and cultured for 4 days. The FcγR-mediated phagocytosis of sensitized erythrocytes (EA) and the complement receptor (CR)-mediated phagocytosis of complement-coated yeast particles were studied during monocyte/macrophage differentiation. Changes in the gene expression of FXIII-A were detected by real-time quantitative RT-PCR. FXIII-A protein production was investigated with fluorescent image analysis at single cell level and Western immunoblot analysis. Both the FcγR and CR-mediated phagocytosis increased during culturing, which peaked on day 3. The phagocytic activity of the cells could be markedly inhibited with monodansylcadaverine, an inhibitor of the transglutaminase-induced crosslinking of proteins. The phagocytosis of EA, complement-coated and uncoated yeast particles was found to be strongly diminished in monocytes of FXIII-A deficient patients. The phagocytic functions of cultured cells showed a change in parallel with the alterations in FXIII-A mRNA expression, as well as with that in FXIII-A in protein synthesis detected by image and Western immunoblot analyses in concert. Our results suggest that FXIII-A plays a role in the Fcγ and complement receptor-mediated phagocytic activities of monocytes/macrophages.",
keywords = "Complement receptor, Factor XIII, Fcγ receptor, Monocyte/macrophage differentiation, Phagocytosis",
author = "Attila S{\'a}rv{\'a}ry and S{\'a}ndor Szucs and Imre Balogh and {\'A}ron Becsky and Helga B{\'a}rdos and M{\'a}ria K{\'a}vai and Uri Seligsohn and Rudolf Egbring and Stanislaw Lopaciuk and L{\'a}szl{\'o} Muszbek and R{\'o}za {\'A}d{\'a}ny",
year = "2004",
month = apr,
doi = "10.1016/j.cellimm.2004.04.007",
language = "אנגלית",
volume = "228",
pages = "81--90",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "2",
}