Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis

Eliora Z. Ron; Robert E. Kohler; Bernard D. Davis

doi:10.1126/science.153.3740.1119

Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis

Eliora Z. Ron^*
, Robert E. Kohler
, Bernard D. Davis

^*Corresponding author for this work

Harvard University

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.

Original language	English
Pages (from-to)	1119-1120
Number of pages	2
Journal	Science
Volume	153
Issue number	3740
DOIs	https://doi.org/10.1126/science.153.3740.1119
State	Published - 1966
Externally published	Yes

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title = "Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis",

abstract = "Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.",

author = "Ron, \{Eliora Z.\} and Kohler, \{Robert E.\} and Davis, \{Bernard D.\}",

year = "1966",

doi = "10.1126/science.153.3740.1119",

language = "אנגלית",

volume = "153",

pages = "1119--1120",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "3740",

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T1 - Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis

AU - Ron, Eliora Z.

AU - Kohler, Robert E.

AU - Davis, Bernard D.

PY - 1966

Y1 - 1966

N2 - Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.

AB - Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.

UR - https://www.scopus.com/pages/publications/0014004365

U2 - 10.1126/science.153.3740.1119

DO - 10.1126/science.153.3740.1119

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C2 - 5331371

AN - SCOPUS:0014004365

SN - 0036-8075

VL - 153

SP - 1119

EP - 1120

JO - Science

JF - Science

IS - 3740

ER -

Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis

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