Poly(dG)-poly(dC) DNA appears shorter than poly(dA)-poly(dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions

Natalia Borovok; Tatiana Molotsky; Jamal Ghabboun; Hezy Cohen; Danny Porath; Alexander Kotlyar

doi:10.1016/j.febslet.2007.11.058

Poly(dG)-poly(dC) DNA appears shorter than poly(dA)-poly(dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions

Natalia Borovok, Tatiana Molotsky, Jamal Ghabboun, Hezy Cohen, Danny Porath^*, Alexander Kotlyar

^*Corresponding author for this work

Biochemistry Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Three types of DNA: ∼2700 bp polydeoxyguanylic olydeoxycytidylic acid [poly(dG)-poly(dC)], ∼2700 bp polydeoxyadenylic polydeoxythymidylic acid [poly(dA)-poly(dT)] and 2686 bp linear plasmid pUC19 were deposited on a mica surface and imaged by atomic force microscopy. Contour length measurements show that the average length of poly(dG)-poly(dC) is ∼30% shorter than that of poly(dA)-poly(dT) and the plasmid. This led us to suggest that individual poly(dG)-poly(dC) molecules are immobilized on mica under ambient conditions in a form which is likely related to the A-form of DNA in contrast to poly(dA)-poly(dT) and random sequence DNA which are immobilized in a form that is related to the DNA B-form.

Original language	English
Pages (from-to)	5843-5846
Number of pages	4
Journal	FEBS Letters
Volume	581
Issue number	30
DOIs	https://doi.org/10.1016/j.febslet.2007.11.058
State	Published - 22 Dec 2007

Keywords

A-form
Atomic force microscopy (AFM)
DNA imaging
Poly(dA)-poly(dT)
Poly(dG)-poly(dC)

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10.1016/j.febslet.2007.11.058

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title = "Poly(dG)-poly(dC) DNA appears shorter than poly(dA)-poly(dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions",

abstract = "Three types of DNA: ∼2700 bp polydeoxyguanylic olydeoxycytidylic acid [poly(dG)-poly(dC)], ∼2700 bp polydeoxyadenylic polydeoxythymidylic acid [poly(dA)-poly(dT)] and 2686 bp linear plasmid pUC19 were deposited on a mica surface and imaged by atomic force microscopy. Contour length measurements show that the average length of poly(dG)-poly(dC) is ∼30% shorter than that of poly(dA)-poly(dT) and the plasmid. This led us to suggest that individual poly(dG)-poly(dC) molecules are immobilized on mica under ambient conditions in a form which is likely related to the A-form of DNA in contrast to poly(dA)-poly(dT) and random sequence DNA which are immobilized in a form that is related to the DNA B-form.",

keywords = "A-form, Atomic force microscopy (AFM), DNA imaging, Poly(dA)-poly(dT), Poly(dG)-poly(dC)",

author = "Natalia Borovok and Tatiana Molotsky and Jamal Ghabboun and Hezy Cohen and Danny Porath and Alexander Kotlyar",

note = "Funding Information: We thank Igor Brodsky for assistance and fruitful discussions. The work was supported by the EC through Contracts IST-2001-38951 (“DNA-Based Nanowires”) and FP6-029192 (“DNA-Based Nanodevices”).",

year = "2007",

month = dec,

day = "22",

doi = "10.1016/j.febslet.2007.11.058",

language = "אנגלית",

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AU - Borovok, Natalia

AU - Molotsky, Tatiana

AU - Ghabboun, Jamal

AU - Cohen, Hezy

AU - Porath, Danny

AU - Kotlyar, Alexander

N1 - Funding Information: We thank Igor Brodsky for assistance and fruitful discussions. The work was supported by the EC through Contracts IST-2001-38951 (“DNA-Based Nanowires”) and FP6-029192 (“DNA-Based Nanodevices”).

PY - 2007/12/22

Y1 - 2007/12/22

N2 - Three types of DNA: ∼2700 bp polydeoxyguanylic olydeoxycytidylic acid [poly(dG)-poly(dC)], ∼2700 bp polydeoxyadenylic polydeoxythymidylic acid [poly(dA)-poly(dT)] and 2686 bp linear plasmid pUC19 were deposited on a mica surface and imaged by atomic force microscopy. Contour length measurements show that the average length of poly(dG)-poly(dC) is ∼30% shorter than that of poly(dA)-poly(dT) and the plasmid. This led us to suggest that individual poly(dG)-poly(dC) molecules are immobilized on mica under ambient conditions in a form which is likely related to the A-form of DNA in contrast to poly(dA)-poly(dT) and random sequence DNA which are immobilized in a form that is related to the DNA B-form.

AB - Three types of DNA: ∼2700 bp polydeoxyguanylic olydeoxycytidylic acid [poly(dG)-poly(dC)], ∼2700 bp polydeoxyadenylic polydeoxythymidylic acid [poly(dA)-poly(dT)] and 2686 bp linear plasmid pUC19 were deposited on a mica surface and imaged by atomic force microscopy. Contour length measurements show that the average length of poly(dG)-poly(dC) is ∼30% shorter than that of poly(dA)-poly(dT) and the plasmid. This led us to suggest that individual poly(dG)-poly(dC) molecules are immobilized on mica under ambient conditions in a form which is likely related to the A-form of DNA in contrast to poly(dA)-poly(dT) and random sequence DNA which are immobilized in a form that is related to the DNA B-form.

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KW - Poly(dG)-poly(dC)

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Poly(dG)-poly(dC) DNA appears shorter than poly(dA)-poly(dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions

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