Plugging into enzymes: Nanowiring of redox enzymes by a gold nanoparticle

Yi Xiao, Fernando Patolsky, Eugenii Katz, James F. Hainfeld, Itamar Willner

Research output: Contribution to journalArticlepeer-review

Abstract

The reconstitution of an apo-flavoenzyme, apo-glucose oxidase, on a 1,4-nanometer gold nanocrystal functionalized with the cofactor flavin adenine dinucleotide and integrated into a conductive film yields a bioelectrocatalytic system with exceptional electrical contact with the electrode support. The electron transfer turnover rate of the reconstituted bioelectrocatalyst is ∼5000 per second, compared with the rate at which molecular oxygen, the natural cosubstrate of the enzyme, accepts electrons (∼700 per second). The gold nanoparticle acts as an electron relay or "electrical nanoplug" for the alignment of the enzyme on the conductive support and for the electrical wiring of its redox-active center.

Original languageEnglish
Pages (from-to)1877-1881
Number of pages5
JournalScience
Volume299
Issue number5614
DOIs
StatePublished - 21 Mar 2003
Externally publishedYes

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