Platelet protection by aprotinin in cardiopulmonary bypass: Electron microscopic study

Jacob Lavee, Naphtali Savion, Aram Smolinsky, Daniel A. Goor*, Rephael Mohr

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

To evaluate the functional integrity of platelets in patients administered the proteinase inhibitor aprotinin during cardiopulmonary bypass, 20 patients undergoing a complicated and prolonged open heart operation were studied. They were randomized to receive either a high dose of aprotinin (total dose, 6 to 7 × 106 KIU) before and during cardiopulmonary bypass (10 patients) or a placebo (10 patients). Blood samples were collected preoperatively, at the termination of bypass, and 90 minutes thereafter to assess platelet count and aggregation on extracellular matrix, which was studied by scanning electron microscopy. On a scale of 1 to 4, mean preoperative platelet aggregation grades were similar in both groups (3.5 ± 0.5). Postoperatively, at the termination of cardiopulmonary bypass and 90 minutes thereafter, all 10 patients treated with aprotinin revealed normal, unchanged platelet aggregation (grade, 3.5 ± 0.5), whereas all placebo-treated patients showed severely disturbed aggregation (grade, 1.4 ± 0.5) (p < 0.001). The platelet count was similar in both groups before and after operation (preoperatively, 182 ± 75 × 109/L and 146 ± 30 × 109/L, and postoperatively, 87 ± 13 × 109/L and 80 ± 27 × 109/L for the aprotinin and placebo groups, respectively). Total 24-hour postoperative bleeding and blood requirement were significantly lower in the aprotinin group (371 ± 84 mL and 2 ± 0.7 units, respectively) compared with the placebo group (608 ± 28 mL and 3.4 ± 1.3 units, respectively) (p < 0.01). These results demonstrate that improved postoperative hemnstatis is directly related to the complete preservation of platelet function achieved by the protective properties of aprotinin.

Original languageEnglish
Pages (from-to)477-481
Number of pages5
JournalAnnals of Thoracic Surgery
Volume53
Issue number3
DOIs
StatePublished - Mar 1992

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