TY - JOUR
T1 - Platelet-activating factor induces progesterone secretion and changes in morphological appearance in luteinizing granulosa cells in vitro
AU - Rabinovici, J.
AU - Angle, M. J.
N1 - Funding Information:
Received September 6, 1990; revised and accepted February 12,1991. * Presented in part at the 37th Annual Meeting of the Society of Gynecological Investigation, St. Louis, Missouri, March 21 to 24,1990. t Recipient of a grant from the Rothchild Foundation (Yad Hanadiv), Jerusalem, Israel, and a Fulbright Scholarship, Washington, D.C. :j: Reprint requests: Jaron Rabinovici, M.D., Reproductive Endocrinology Center, Room HSW-1697, Box 0556, University of California San Francisco, San Francisco, California 94143. § Present address: IVF Program, Women's Hospital, Las Vegas, Nevada.
PY - 1991
Y1 - 1991
N2 - Objective: Recent evidence indicates the involvement of platelet-activating factor (PAF) in ovarian periovulatory processes. The objective of this study was to investigate the influence of PAF on ovarian follicular cellular function. Design and Interventions: We examined the effects of PAF on progesterone (P) secretion by cultured human luteinizing granulosa cells obtained from women undergoing in vitro fertilization. Follicular cells were cultured in serum-free conditions for 12 to 36 hours with and without PAF (at dosages of 10-8 to 10-14 M). Results: Progesterone secretion was significantly increased at 12 and 36 hours after treatment with 10-8 M PAF (170% ± 40% and 210% ± 40%, respectively) and with 10-10 M PAF (170% ± 30% and 180% ± 10%, respectively). Smaller increases were seen with 10-12 and 10-14 M PAF. Maximal increases in P secretion in 10-10 M PAF-treated wells occurred at 3 to 12 hours. At 36 hours, distinct morphological differences between cells in control wells and those containing PAF were noted. Conclusions: These results indicate that PAF may influence follicular cell function during ovulation and corpus luteum formation.
AB - Objective: Recent evidence indicates the involvement of platelet-activating factor (PAF) in ovarian periovulatory processes. The objective of this study was to investigate the influence of PAF on ovarian follicular cellular function. Design and Interventions: We examined the effects of PAF on progesterone (P) secretion by cultured human luteinizing granulosa cells obtained from women undergoing in vitro fertilization. Follicular cells were cultured in serum-free conditions for 12 to 36 hours with and without PAF (at dosages of 10-8 to 10-14 M). Results: Progesterone secretion was significantly increased at 12 and 36 hours after treatment with 10-8 M PAF (170% ± 40% and 210% ± 40%, respectively) and with 10-10 M PAF (170% ± 30% and 180% ± 10%, respectively). Smaller increases were seen with 10-12 and 10-14 M PAF. Maximal increases in P secretion in 10-10 M PAF-treated wells occurred at 3 to 12 hours. At 36 hours, distinct morphological differences between cells in control wells and those containing PAF were noted. Conclusions: These results indicate that PAF may influence follicular cell function during ovulation and corpus luteum formation.
UR - https://www.scopus.com/pages/publications/0025832557
U2 - 10.1016/S0015-0282(16)54360-X
DO - 10.1016/S0015-0282(16)54360-X
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C2 - 2037105
AN - SCOPUS:0025832557
SN - 0015-0282
VL - 55
SP - 1106
EP - 1111
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 6
ER -