TY - JOUR
T1 - Plasma dependent reduction in red blood cell aggregation after dextran sulfate low-density lipoprotein apheresis - Implications for rheological studies
AU - Schechner, Vered
AU - Ben-Ami, Ronen
AU - Hershcovici, Tiberiu
AU - Yedgar, Shaul
AU - Beigel, Ytzhak
AU - Shapira, Itzhak
AU - Berliner, Shlomo
AU - Barsthein, Gershon
PY - 2005/10
Y1 - 2005/10
N2 - Red blood cell (RBC) aggregation is increased in familial hypercholesterolemia, and is reduced significantly after low density lipoprotein (LDL) apheresis. The purpose of the present study was to clarify whether this reduction depends on changes in plasma composition, RBC membrane properties, or both. RBC aggregation was determined in a computerized cell flow-properties analyzer, before and after LDL apheresis. We compared RBC aggregation in autologous plasma with aggregation in a plasma-free standard solution (0.5% of dextran 500 kDa) to define the separate contributions of plasma and cellular properties to the observed RBC aggregation. RBC aggregation in autologous plasma was reduced by 35.5% after LDL apheresis (P = 0.01) but was not significantly affected when measured in dextran 500. This suggests that LDL apheresis attenuated RBC aggregation by altering plasma composition rather than RBC membrane properties. These results are relevant to the understanding of hemorheological changes which follow therapeutic apheresis in hypercholesterolemic patients.
AB - Red blood cell (RBC) aggregation is increased in familial hypercholesterolemia, and is reduced significantly after low density lipoprotein (LDL) apheresis. The purpose of the present study was to clarify whether this reduction depends on changes in plasma composition, RBC membrane properties, or both. RBC aggregation was determined in a computerized cell flow-properties analyzer, before and after LDL apheresis. We compared RBC aggregation in autologous plasma with aggregation in a plasma-free standard solution (0.5% of dextran 500 kDa) to define the separate contributions of plasma and cellular properties to the observed RBC aggregation. RBC aggregation in autologous plasma was reduced by 35.5% after LDL apheresis (P = 0.01) but was not significantly affected when measured in dextran 500. This suggests that LDL apheresis attenuated RBC aggregation by altering plasma composition rather than RBC membrane properties. These results are relevant to the understanding of hemorheological changes which follow therapeutic apheresis in hypercholesterolemic patients.
KW - Erythrocyte aggregation
KW - Low density lipoprotein apheresis
KW - Plasma
UR - http://www.scopus.com/inward/record.url?scp=33645453017&partnerID=8YFLogxK
U2 - 10.1111/j.1744-9987.2005.00317.x
DO - 10.1111/j.1744-9987.2005.00317.x
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AN - SCOPUS:33645453017
SN - 1744-9979
VL - 9
SP - 379
EP - 384
JO - Therapeutic Apheresis and Dialysis
JF - Therapeutic Apheresis and Dialysis
IS - 5
ER -