TY - CHAP
T1 - Plasma biomarker identification and quantification by microparticle proteomics
AU - Harel, Michal
AU - Geiger, Tamar
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media LLC.
PY - 2017
Y1 - 2017
N2 - Plasma biomarker discovery necessitates a method for deep proteomic profiling, as well as for highly accurate quantification of the proteins in the sample. Furthermore, to obtain strong candidates for potential biomarkers, the method should be high throughput to enable a large scale analysis. Here we describe in detail PROMIS-Quan (PROteomics of MIcroparticles using Super-SILAC Quantification), a method for a simple and robust fractionation of the plasma samples by extraction of plasma microparticles, followed by SILAC-based relative and absolute quantification.
AB - Plasma biomarker discovery necessitates a method for deep proteomic profiling, as well as for highly accurate quantification of the proteins in the sample. Furthermore, to obtain strong candidates for potential biomarkers, the method should be high throughput to enable a large scale analysis. Here we describe in detail PROMIS-Quan (PROteomics of MIcroparticles using Super-SILAC Quantification), a method for a simple and robust fractionation of the plasma samples by extraction of plasma microparticles, followed by SILAC-based relative and absolute quantification.
KW - Absolute quantification
KW - Extracellular vesicles
KW - Microparticles
KW - Plasma biomarker discovery
KW - Plasma fractionation
KW - Relative quantification
KW - Super-SILAC quantification
UR - http://www.scopus.com/inward/record.url?scp=85021715611&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7057-5_33
DO - 10.1007/978-1-4939-7057-5_33
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C2 - 28674905
AN - SCOPUS:85021715611
T3 - Methods in Molecular Biology
SP - 477
EP - 486
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -