Plant virus movement protein dynamics probed with a GFP-protein fusion

Bernard L. Epel, Hal S. Padgett, Manfred Heinlein, Roger N. Beachy

Research output: Contribution to journalArticlepeer-review

Abstract

A genetic fusion between the gene encoding green fluorescent protein (GFP) from the jellyfish Aequorea victoria, with that of the Ob-tobamovirus movement protein (MP) resulted in the expression of a fluorescent fusion protein (MP::GFP) that was fully biologically active in mediating the cell-to-cell spread of the Ob-virus. The MP::GFP fusion was used to follow in planta the subcellular trafficking of MP. GFP-tagged MP was transiently expressed and found to be associated with several subcellular compartments and structures including trans-wall structures, presumably plasmodesmata, and filament structures. The MP::GFP fusion can be used to monitor MP association with host proteins and structures, and for the isolation of interacting host components.

Original languageEnglish
Pages (from-to)75-79
Number of pages5
JournalGene
Volume173
Issue number1
DOIs
StatePublished - 1996

Keywords

  • Green fluorescent protein
  • Pathogenesis
  • Reporter protein
  • TMV
  • Tobamovirus
  • Virus movement

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