Plant virus movement protein dynamics probed with a GFP-protein fusion

Bernard L. Epel; Hal S. Padgett; Manfred Heinlein; Roger N. Beachy

doi:10.1016/0378-1119(95)00678-8

Plant virus movement protein dynamics probed with a GFP-protein fusion

Bernard L. Epel, Hal S. Padgett, Manfred Heinlein, Roger N. Beachy

School of Plant Sciences and Food Security

Research output: Contribution to journal › Article › peer-review

Abstract

A genetic fusion between the gene encoding green fluorescent protein (GFP) from the jellyfish Aequorea victoria, with that of the Ob-tobamovirus movement protein (MP) resulted in the expression of a fluorescent fusion protein (MP::GFP) that was fully biologically active in mediating the cell-to-cell spread of the Ob-virus. The MP::GFP fusion was used to follow in planta the subcellular trafficking of MP. GFP-tagged MP was transiently expressed and found to be associated with several subcellular compartments and structures including trans-wall structures, presumably plasmodesmata, and filament structures. The MP::GFP fusion can be used to monitor MP association with host proteins and structures, and for the isolation of interacting host components.

Original language	English
Pages (from-to)	75-79
Number of pages	5
Journal	Gene
Volume	173
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(95)00678-8
State	Published - 1996

Keywords

Green fluorescent protein
Pathogenesis
Reporter protein
TMV
Tobamovirus
Virus movement

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10.1016/0378-1119(95)00678-8

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@article{28dd24f6be16490da383708601303fdd,

title = "Plant virus movement protein dynamics probed with a GFP-protein fusion",

abstract = "A genetic fusion between the gene encoding green fluorescent protein (GFP) from the jellyfish Aequorea victoria, with that of the Ob-tobamovirus movement protein (MP) resulted in the expression of a fluorescent fusion protein (MP::GFP) that was fully biologically active in mediating the cell-to-cell spread of the Ob-virus. The MP::GFP fusion was used to follow in planta the subcellular trafficking of MP. GFP-tagged MP was transiently expressed and found to be associated with several subcellular compartments and structures including trans-wall structures, presumably plasmodesmata, and filament structures. The MP::GFP fusion can be used to monitor MP association with host proteins and structures, and for the isolation of interacting host components.",

keywords = "Green fluorescent protein, Pathogenesis, Reporter protein, TMV, Tobamovirus, Virus movement",

author = "Epel, {Bernard L.} and Padgett, {Hal S.} and Manfred Heinlein and Beachy, {Roger N.}",

note = "Funding Information: This work was supported by National Science Foundationg rantsMCB 9209530a nd MCB 9317368t o R.N.B., and by fellowshipsf rom the Human Frontiers Science Program and the Deutsche Forschungs-gemeinschaf(tD FG) to M.H. We are gratefult o Dr. R. Tsien (Universityo f California, San Diego, CA, USA), and Dr. Jeff Harper, TSRI, for the modified GFP used in the cloning reactions.",

year = "1996",

doi = "10.1016/0378-1119(95)00678-8",

language = "אנגלית",

volume = "173",

pages = "75--79",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Plant virus movement protein dynamics probed with a GFP-protein fusion

AU - Epel, Bernard L.

AU - Padgett, Hal S.

AU - Heinlein, Manfred

AU - Beachy, Roger N.

N1 - Funding Information: This work was supported by National Science Foundationg rantsMCB 9209530a nd MCB 9317368t o R.N.B., and by fellowshipsf rom the Human Frontiers Science Program and the Deutsche Forschungs-gemeinschaf(tD FG) to M.H. We are gratefult o Dr. R. Tsien (Universityo f California, San Diego, CA, USA), and Dr. Jeff Harper, TSRI, for the modified GFP used in the cloning reactions.

PY - 1996

Y1 - 1996

N2 - A genetic fusion between the gene encoding green fluorescent protein (GFP) from the jellyfish Aequorea victoria, with that of the Ob-tobamovirus movement protein (MP) resulted in the expression of a fluorescent fusion protein (MP::GFP) that was fully biologically active in mediating the cell-to-cell spread of the Ob-virus. The MP::GFP fusion was used to follow in planta the subcellular trafficking of MP. GFP-tagged MP was transiently expressed and found to be associated with several subcellular compartments and structures including trans-wall structures, presumably plasmodesmata, and filament structures. The MP::GFP fusion can be used to monitor MP association with host proteins and structures, and for the isolation of interacting host components.

AB - A genetic fusion between the gene encoding green fluorescent protein (GFP) from the jellyfish Aequorea victoria, with that of the Ob-tobamovirus movement protein (MP) resulted in the expression of a fluorescent fusion protein (MP::GFP) that was fully biologically active in mediating the cell-to-cell spread of the Ob-virus. The MP::GFP fusion was used to follow in planta the subcellular trafficking of MP. GFP-tagged MP was transiently expressed and found to be associated with several subcellular compartments and structures including trans-wall structures, presumably plasmodesmata, and filament structures. The MP::GFP fusion can be used to monitor MP association with host proteins and structures, and for the isolation of interacting host components.

KW - Green fluorescent protein

KW - Pathogenesis

KW - Reporter protein

KW - TMV

KW - Tobamovirus

KW - Virus movement

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DO - 10.1016/0378-1119(95)00678-8

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AN - SCOPUS:0030008433

VL - 173

SP - 75

EP - 79

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -

Plant virus movement protein dynamics probed with a GFP-protein fusion

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Keywords

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