@article{28dd24f6be16490da383708601303fdd,
title = "Plant virus movement protein dynamics probed with a GFP-protein fusion",
abstract = "A genetic fusion between the gene encoding green fluorescent protein (GFP) from the jellyfish Aequorea victoria, with that of the Ob-tobamovirus movement protein (MP) resulted in the expression of a fluorescent fusion protein (MP::GFP) that was fully biologically active in mediating the cell-to-cell spread of the Ob-virus. The MP::GFP fusion was used to follow in planta the subcellular trafficking of MP. GFP-tagged MP was transiently expressed and found to be associated with several subcellular compartments and structures including trans-wall structures, presumably plasmodesmata, and filament structures. The MP::GFP fusion can be used to monitor MP association with host proteins and structures, and for the isolation of interacting host components.",
keywords = "Green fluorescent protein, Pathogenesis, Reporter protein, TMV, Tobamovirus, Virus movement",
author = "Epel, {Bernard L.} and Padgett, {Hal S.} and Manfred Heinlein and Beachy, {Roger N.}",
note = "Funding Information: This work was supported by National Science Foundationg rantsMCB 9209530a nd MCB 9317368t o R.N.B., and by fellowshipsf rom the Human Frontiers Science Program and the Deutsche Forschungs-gemeinschaf(tD FG) to M.H. We are gratefult o Dr. R. Tsien (Universityo f California, San Diego, CA, USA), and Dr. Jeff Harper, TSRI, for the modified GFP used in the cloning reactions.",
year = "1996",
doi = "10.1016/0378-1119(95)00678-8",
language = "אנגלית",
volume = "173",
pages = "75--79",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier B.V.",
number = "1",
}