Plant regeneration from immature and mature embryo derived calli of Hordeum marinum

Daphne Rotem-Abarbanell, Adina Breiman

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Callus cultures were obtained from immature and mature embryos of Hordeum marnium on MS media containing 0.5 mg l-1 parachlorophenoxyacetic acid or 2 mg l-1 2,4-dichlorophenoxyacetic acid. Regeneration occurred after transferring calli to MS either devoid of hormones or supplemented with 1 mg l-1 indole-3-acetic acid and 1 mg l-1 zeatin. The regeneration capacity of the immature embryo derived calli (94%) was about 5 times higher than that of mature embryo derived calli (17%). A total of 30 and 964 plantlets were obtained from 21 mature and 59 immature embryo derived calli, respectively. Low frequency (less than 1%) of albino plantlets was obtained from both explants after 3-9 months in culture. Plants expressing transient chlorophyll deficiency were produced from immature embryo derived cultures at a frequency of 10%. However, when transferred to soil, these plantlets became green.

Original languageEnglish
Pages (from-to)207-216
Number of pages10
JournalPlant Cell, Tissue and Organ Culture
Issue number3
StatePublished - Mar 1989


  • Hordeum marinum
  • immature and mature embryo derived calli
  • plant regeneration
  • wild barley


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