TY - JOUR
T1 - Phytohemagglutinin skin test
T2 - A possible in vivo measure of cell-mediated immunity
AU - Bonforte, Richard J.
AU - Topilsky, Marcel
AU - Siltzbach, Louis E.
AU - Glade, Philip R.
N1 - Funding Information:
From the Division of Infectious Diseases of the Department of Pediatrics and the Division of Thoracic Diseases of the Department of Medicine of The Mount Sinai School of Medicine of The City University of New York. Supported by Research Contract NIH-69-2078 with the Special Virus Cancer program of the National Cancer Institute, National Institutes of Health, United States Public Health Service, by Research Grant HE-13853-12A1 #ore the National Heart and Lung Institute, and by Research Grant RO1-A110422 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Public Health Service. Presented in part at the annual meeting of the Society for Pediatric Research, Atlantic City, N. \]., April, 1971. Reprint address: Richard \]. Bonforte, M.D., Department of Pediatrics, Division of Inlectious Diseases, Mount Sinai School of Medicine, tOOth St. and Filth Ave., New York N. Y. 10029. ~Reclpient of a National Institutes of Health training grant in pediatric research, No. HD 00243-04. ~Recipient of a Research Career Development Award (A1-46371) of the United States Public Health Service.
PY - 1972/10
Y1 - 1972/10
N2 - The usefulness of in vivo skin reactivity to phytohemagglutinin to assess cell-mediated immunity was investigated in 25 individuals without disease, including ten premature infants. Intradermal injection of 2 μg of phytohemagglutinin produced erythema and induration within 24 to 48 hours. Cutaneous reactivity to phytohemagglutinin was present in the premature infants despite absence of response to intradermal monilia extract and to intradermal streptokinase-streptodornase (SK-SD). In 14 of 16 patients with sarcoidosis and two of three patients with congenital rubella syndrome-diseases associated with altered cell-mediated immunity-2 μg of phytohemagglutinin intracutaneously produced erythema and induration at 48 hours despite the presence or absence of cutaneous reactivity to other skin test antigens. Patients with sarcoidosis responded to phytohemagglutinin in vivo and in vitro, although their in vitro responses were uniformly depressed.
AB - The usefulness of in vivo skin reactivity to phytohemagglutinin to assess cell-mediated immunity was investigated in 25 individuals without disease, including ten premature infants. Intradermal injection of 2 μg of phytohemagglutinin produced erythema and induration within 24 to 48 hours. Cutaneous reactivity to phytohemagglutinin was present in the premature infants despite absence of response to intradermal monilia extract and to intradermal streptokinase-streptodornase (SK-SD). In 14 of 16 patients with sarcoidosis and two of three patients with congenital rubella syndrome-diseases associated with altered cell-mediated immunity-2 μg of phytohemagglutinin intracutaneously produced erythema and induration at 48 hours despite the presence or absence of cutaneous reactivity to other skin test antigens. Patients with sarcoidosis responded to phytohemagglutinin in vivo and in vitro, although their in vitro responses were uniformly depressed.
UR - http://www.scopus.com/inward/record.url?scp=0015416494&partnerID=8YFLogxK
U2 - 10.1016/S0022-3476(72)80101-X
DO - 10.1016/S0022-3476(72)80101-X
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AN - SCOPUS:0015416494
SN - 0022-3476
VL - 81
SP - 775
EP - 780
JO - Journal of Pediatrics
JF - Journal of Pediatrics
IS - 4
ER -