TY - JOUR
T1 - Phytochrome B regulates reactive oxygen signaling during abiotic and biotic stress in plants
AU - Fichman, Yosef
AU - Xiong, Haiyan
AU - Sengupta, Soham
AU - Morrow, Johanna
AU - Loog, Hailey
AU - Azad, Rajeev K.
AU - Hibberd, Julian M.
AU - Liscum, Emmanuel
AU - Mittler, Ron
N1 - Publisher Copyright:
© 2022 The Authors New Phytologist © 2022 New Phytologist Foundation.
PY - 2023/3
Y1 - 2023/3
N2 - Reactive oxygen species (ROS) and the photoreceptor protein phytochrome B (phyB) play a key role in plant acclimation to stress. However, how phyB that primarily functions in the nuclei impacts ROS signaling mediated by respiratory burst oxidase homolog (RBOH) proteins that reside on the plasma membrane, during stress, is unknown. Arabidopsis thaliana and Oryza sativa mutants, RNA-Seq, bioinformatics, biochemistry, molecular biology, and whole-plant ROS imaging were used to address this question. Here, we reveal that phyB and RBOHs function as part of a key regulatory module that controls apoplastic ROS production, stress-response transcript expression, and plant acclimation in response to excess light stress. We further show that phyB can regulate ROS production during stress even if it is restricted to the cytosol and that phyB, respiratory burst oxidase protein D (RBOHD), and respiratory burst oxidase protein F (RBOHF) coregulate thousands of transcripts in response to light stress. Surprisingly, we found that phyB is also required for ROS accumulation in response to heat, wounding, cold, and bacterial infection. Our findings reveal that phyB plays a canonical role in plant responses to biotic and abiotic stresses, regulating apoplastic ROS production, possibly while at the cytosol, and that phyB and RBOHD/RBOHF function in the same regulatory pathway.
AB - Reactive oxygen species (ROS) and the photoreceptor protein phytochrome B (phyB) play a key role in plant acclimation to stress. However, how phyB that primarily functions in the nuclei impacts ROS signaling mediated by respiratory burst oxidase homolog (RBOH) proteins that reside on the plasma membrane, during stress, is unknown. Arabidopsis thaliana and Oryza sativa mutants, RNA-Seq, bioinformatics, biochemistry, molecular biology, and whole-plant ROS imaging were used to address this question. Here, we reveal that phyB and RBOHs function as part of a key regulatory module that controls apoplastic ROS production, stress-response transcript expression, and plant acclimation in response to excess light stress. We further show that phyB can regulate ROS production during stress even if it is restricted to the cytosol and that phyB, respiratory burst oxidase protein D (RBOHD), and respiratory burst oxidase protein F (RBOHF) coregulate thousands of transcripts in response to light stress. Surprisingly, we found that phyB is also required for ROS accumulation in response to heat, wounding, cold, and bacterial infection. Our findings reveal that phyB plays a canonical role in plant responses to biotic and abiotic stresses, regulating apoplastic ROS production, possibly while at the cytosol, and that phyB and RBOHD/RBOHF function in the same regulatory pathway.
KW - acclimation
KW - phytochrome
KW - reactive oxygen species
KW - respiratory burst oxidase homolog
KW - signal transduction
KW - stress
UR - http://www.scopus.com/inward/record.url?scp=85144058859&partnerID=8YFLogxK
U2 - 10.1111/nph.18626
DO - 10.1111/nph.18626
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 36401805
AN - SCOPUS:85144058859
SN - 0028-646X
VL - 237
SP - 1711
EP - 1727
JO - New Phytologist
JF - New Phytologist
IS - 5
ER -