Physiological expression of mutated TAU impaired astrocyte activity and exacerbates β-amyloid pathology in 5xFAD mice

Dorit Farfara, Meital Sooliman, Limor Avrahami, Tabitha Grace Royal, Shoshik Amram, Lea Rozenstein-Tsalkovich, Dorit Trudler, Shani Blanga-Kanfi, Hagit Eldar-Finkelman, Jens Pahnke*, Hanna Rosenmann*, Dan Frenkel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background: Alzheimer’s disease (AD) is the leading cause of dementia in the world. The pathology of AD is affiliated with the elevation of both tau (τ) and β-amyloid (Aβ) pathologies. Yet, the direct link between natural τ expression on glia cell activity and Aβ remains unclear. While experiments in mouse models suggest that an increase in Aβ exacerbates τ pathology when expressed under a neuronal promoter, brain pathology from AD patients suggests an appearance of τ pathology in regions without Aβ. Methods: Here, we aimed to assess the link between τ and Aβ using a new mouse model that was generated by crossing a mouse model that expresses two human mutations of the human MAPT under a mouse Tau natural promoter with 5xFAD mice that express human mutated APP and PS1 in neurons. Results: The new mouse model, called 5xFAD TAU, shows accelerated cognitive impairment at 2 months of age, increased number of Aβ depositions at 4 months and neuritic plaques at 6 months of age. An expression of human mutated TAU in astrocytes leads to a dystrophic appearance and reduces their ability to engulf Aβ, which leads to an increased brain Aβ load. Astrocytes expressing mutated human TAU showed an impairment in the expression of vascular endothelial growth factor (VEGF) that has previously been suggested to play an important role in supporting neurons. Conclusions: Our results suggest the role of τ in exacerbating Aβ pathology in addition to pointing out the potential role of astrocytes in disease progression. Further research of the crosstalk between τ and Aβ in astrocytes may increase our understanding of the role glia cells have in the pathology of AD with the aim of identifying novel therapeutic interventions to an otherwise currently incurable disease.

Original languageEnglish
Article number174
JournalJournal of Neuroinflammation
Volume20
Issue number1
DOIs
StatePublished - Dec 2023

Funding

FundersFunder number
Deutsche Forschungsgemeinschaft/Germany16154, DFG 263024513
HelseSØTAČR TARIMAD TO01000078, 2019054, 2019055, 2022046, 19008
Norges forskningsrådet/Norway295910, 327571
University of Texas Medical Branch
Horizon 2020 Framework ProgrammeJPco-fuND, 643417
EU Joint Programme – Neurodegenerative Disease Research
Agence Nationale de la Recherche2020-02905
Ministerstvo Školství, Mládeže a Tělovýchovy8F21002, RTD/2020/26
Bundesministerium für Bildung und Forschung01ED1605, 260786, 30000-12631, 2015-06795
Israel Science Foundation300000-4895
Österreichische Forschungsförderungsgesellschaft882717, 01ED2106
Universitetet i Oslo
Ministry of Health, State of Israel301228

    Keywords

    • 5xFAD
    • Alzheimer’s disease
    • Astrocytes
    • Beta-amyloid
    • Mouse model
    • Tau
    • Tauopathy

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