Photoactivated aflatoxins are mutagens to salmonella typhimurium and bind covalently to DNA in vitro

Hagar Israel-kalinsky, Jacob Tuch, Joseph Roitelman, Avishay Abraham Stark

Research output: Contribution to journalArticlepeer-review

Abstract

The mutagenesis of Salmonella typhimurium TA100 and covalent binding in vitro of photoactivated aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2) were investigated. Covalent binding levels of 1140, 225, 330 and 8 pmol aflatoxin per μmol nucleotide phosphate were obtained for AFB1, AFG1, AFB2 and AFG2, respectively, at 50 μM mycotoxin after 2 h of irradiation. Mutant frequencies to histidine prototrophy were 97, 19, 49 and 0 × 10-6 for AFB1, AFG1, AFB2 and AFG2 respectively, after 2 h irradiation at 100 μM mycotoxin in the surviving fraction of the mutagenized cultures. Toxicity to Salmonella was 0.59, 0.03, 0.31 and 0 lethal hits under the conditions specified for mutagenesis for AFB1, AFG1, AFB2 and AFG2, respectively.

Original languageEnglish
Pages (from-to)423-429
Number of pages7
JournalCarcinogenesis
Volume3
Issue number4
DOIs
StatePublished - 1982

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