TY - JOUR
T1 - Photoactivated aflatoxins are mutagens to salmonella typhimurium and bind covalently to DNA in vitro
AU - Israel-kalinsky, Hagar
AU - Tuch, Jacob
AU - Roitelman, Joseph
AU - Stark, Avishay Abraham
N1 - Funding Information:
We are grateful to Dr. D.P.H. Hsieh, Department of Toxicology, University of California, Davis, for AFLH,and AFQ,, and to Dr. B.Z. Fuchs, Department of Chemistry, Tel Aviv University, and Dr. D.F. Liberman, Environmental Medical Service, M.I.T., Cambridge, MA for helpful discussions. This work was supported by a grant from the National Academy of Science in Israel.
PY - 1982
Y1 - 1982
N2 - The mutagenesis of Salmonella typhimurium TA100 and covalent binding in vitro of photoactivated aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2) were investigated. Covalent binding levels of 1140, 225, 330 and 8 pmol aflatoxin per μmol nucleotide phosphate were obtained for AFB1, AFG1, AFB2 and AFG2, respectively, at 50 μM mycotoxin after 2 h of irradiation. Mutant frequencies to histidine prototrophy were 97, 19, 49 and 0 × 10-6 for AFB1, AFG1, AFB2 and AFG2 respectively, after 2 h irradiation at 100 μM mycotoxin in the surviving fraction of the mutagenized cultures. Toxicity to Salmonella was 0.59, 0.03, 0.31 and 0 lethal hits under the conditions specified for mutagenesis for AFB1, AFG1, AFB2 and AFG2, respectively.
AB - The mutagenesis of Salmonella typhimurium TA100 and covalent binding in vitro of photoactivated aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2) were investigated. Covalent binding levels of 1140, 225, 330 and 8 pmol aflatoxin per μmol nucleotide phosphate were obtained for AFB1, AFG1, AFB2 and AFG2, respectively, at 50 μM mycotoxin after 2 h of irradiation. Mutant frequencies to histidine prototrophy were 97, 19, 49 and 0 × 10-6 for AFB1, AFG1, AFB2 and AFG2 respectively, after 2 h irradiation at 100 μM mycotoxin in the surviving fraction of the mutagenized cultures. Toxicity to Salmonella was 0.59, 0.03, 0.31 and 0 lethal hits under the conditions specified for mutagenesis for AFB1, AFG1, AFB2 and AFG2, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0020322192&partnerID=8YFLogxK
U2 - 10.1093/carcin/3.4.423
DO - 10.1093/carcin/3.4.423
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AN - SCOPUS:0020322192
SN - 0143-3334
VL - 3
SP - 423
EP - 429
JO - Carcinogenesis
JF - Carcinogenesis
IS - 4
ER -
