Phospholipids of a moderately halophilic halotolerant bacterium. Isolation and identification of glucosylphosphatidyl-glycerol

Edna Peleg*, Alisa Tietz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

1. 1. The composition of the polar lipids of a Gram-negative, moderately halophilic halotolerant bacterium was determined. The following lipids (in decreasing amounts) were found: phosphatidylethanolamine, phosphatidylglycerol, a glucuronic acid containing glycolipid, α-glucosylphosphatidylglycerol and cardiolipin. 2. 2. The elucidation of the structure of glucosylphosphatidylglycerol is based on the following observations: (a) Degradation by phospholipase C yielded diglycerides and a water-soluble product which upon add hydrolysis yielded glucose and glycerol phosphate, (b) Hydrolysis with 60% HF yielded diglycerides, inorganic phosphate, glucosylglycerol and small amounts of the water-soluble product which was obtained by phospholipase C. (c) Glucosylglycerol was hydrolyzed by α-glucosidase. 3. 3. The fatty acids of these lipids had an unusually high content of 11,12-methylene octadecanoic acid which was preferentially located at carbon 2 of the phosphatidyl unit.

Original languageEnglish
Pages (from-to)368-379
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume306
Issue number3
DOIs
StatePublished - 21 Jun 1973

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