Phosphoinositide breakdown in isolated rat parotid membranes. Stimulation by cholinergic and α-adrenergic agonists

E. Nadler, M. S. Nijjar, Y. Oron

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Parotid gland membranes labelled with [3H]inositol were challenged with the cholinergic agonist, carbamylcholine, or with epinephrine in the presence of propranolol. Both agonists caused a significant breakdown of labelled phosphoinositides (17.5%) in membranes suspended in Krebs-Ringer bicarbonate buffer. This effect was abolished by the respective antagonists, atropine or phentolamine. The carbamylcholine-induced breakdown of labelled phosphoinositides did not require cytosol. The addition of cytosol alone, or the exposure of membranes to a medium of low ionic strength caused a significant breakdown of phosphoinositides (10-40%). No further breakdown due to the addition of carbamylcholine was observed under these conditions. It is suggested that neurotransmitter-induced breakdown of phosphoinositides is effected by membrane-associated enzyme(s) and can be observed only in a medium of high ionic strength.

Original languageEnglish
Pages (from-to)278-282
Number of pages5
JournalFEBS Letters
Volume178
Issue number2
DOIs
StatePublished - 10 Dec 1984

Funding

FundersFunder number
Recanati Foundation for Medical Researcha
SchreiberF oundation
Tel Aviv University

    Keywords

    • Cholinergic receptor
    • Parotid membrane
    • Phosphoinositide breakdown

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