Phosphate metabolites in lymphoid, Friend erythroleukemia, and HeLa cells observed by high-resolution 31P nuclear magnetic resonance

G. Navon, R. Navon, R. G. Shulman, T. Yamane

Research output: Contribution to journalArticlepeer-review

Abstract

High-resolution phosphorus-31 nuclear magnetic resonance spectra were observed for cell lines from mammalian sources: lymphoid cells, Friend erythroleukemia cells, and HeLa cells. Some phosphate metabolites were identified and their concentrations were found to vary among the different cell lines. ATP/ADP ratios could be measured in the living cells. The phospholipid metabolites phosphorylcholine and phosphorylethanolamine and their glyceryl esters were present in relatively high concentrations in the cells, but their ratios varied considerably. Also, a high concentration of an unidentified diphosphodiester compound was observed in the Friend erythroleukemia and HeLa cells. No appreciable pH gradient across the cell membrane was observed in these three mammalian cell lines, in contrast to the large pH gradient detected in microorganisms.

Original languageEnglish
Pages (from-to)891-895
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume75
Issue number2
DOIs
StatePublished - 1978
Externally publishedYes

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