Phenotypic Properties of 3T3 Cells Transformed in vitro with Polyoma Virus and Passaged Once in Syngeneic Animals

Adit Ben Baruch Langer, Noam Emmanuel, Jos Even, Wolf H. Fridman, Ofra Gohar, Bosmat Gonen, Ben Zion Katz, Maya Ran, Nechama Smorodinsky, Isaac Witz

Research output: Contribution to journalArticlepeer-review

Abstract

Cloned BALB/c 3T3 cells transformed in vitro with polyoma virus (PyV) acquired a higher tumorigenicity phenotype after a single in vivo passage. Some of the in vivo passaged cells (CTC cells) exhibited also a higher metastatic phenotype than cells from the same clones that were maintained only in culture (C cells). A phenotypic comparison between CTC and C cells was performed. It was found that most CTC lines exhibited a higher binding to laminin compared to their clonal C cell ancestors. Some CTC cells were less sensitive to the cytotoxic effects of TNF-α than the corresponding C cells. CTC cells originating from tumors which appeared after a long latency period (late tumors) tended to express FcγRII while CTC cells originating from tumors which appeared after a short latency period (early tumors) as well as the corresponding C cells tended not to express FcγRII. The expression of a membrane epitope recognized by a monoclonal antibody expressing specificity towards PyV transformed cells, was down-regulated on late tumor cells compared to early tumor cells. Transfection of cloned PyV-transformed BALB/c 3T3 cells with the β1FcγRII gene augmented the tumorigenicity and metastatic phenotype of the transfectants compared to control transfectants.

Original languageEnglish
Pages (from-to)281-291
Number of pages11
JournalImmunobiology
Volume185
Issue number2-4
DOIs
StatePublished - 1992

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