PHB3 Maintains Root Stem Cell Niche Identity through ROS-Responsive AP2/ERF Transcription Factors in Arabidopsis

Xiangpei Kong, Huiyu Tian, Qianqian Yu, Feng Zhang, Rong Wang, Shan Gao, Wenhong Xu, Jiajia Liu, Eilon Shani, Chunxiang Fu, Gongke Zhou, Liangran Zhang, Xiansheng Zhang, Zhaojun Ding*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

123 Scopus citations

Abstract

The root stem cell niche, which is composed of four mitotically inactive quiescent center (QC) cells and the surrounding actively divided stem cells in Arabidopsis, is critical for growth and root development. Here, we demonstrate that the Arabidopsis prohibitin protein PHB3 is required for the maintenance of root stem cell niche identity by both inhibiting proliferative processes in the QC and stimulating cell division in the proximal meristem (PM). PHB3 coordinates cell division and differentiation in the root apical meristem by restricting the spatial expression of ethylene response factor (ERF) transcription factors 115, 114, and 109. ERF115, ERF114, and ERF109 mediate ROS signaling, in a PLT-independent manner, to control root stem cell niche maintenance and root growth through phytosulfokine (PSK) peptide hormones in Arabidopsis. Prohibitins (PHBs) have been identified as negative regulators of the cell cycle. Here, Kong et al. demonstrate that Arabidopsis PHB3 is essential to maintain QC and DSC identity, and it controls root meristem activity by restricting the spatial expression of the ROS-responsive factors ERF115, 114, and 109 in root.

Original languageEnglish
Pages (from-to)1350-1363
Number of pages14
JournalCell Reports
Volume22
Issue number5
DOIs
StatePublished - 30 Jan 2018

Funding

FundersFunder number
Shandong Province Natural Science Foundation of Major Basic Research Program2017C03
Shandong Provincial Funds for Distinguished Young ScholarsJQ201605, JQ201408
Taishan Scholar Program of Shandong
Fundamental Research Fund of Shandong University2016GN021
National Natural Science Foundation of China31470371, 31500227, 31671293, 31670275
Ministry of Science and Technology of the People's Republic of China2015CB942901
China Postdoctoral Science Foundation2014T70631, 2013M540543

    Keywords

    • ERF
    • PSK
    • ROS
    • prohibitin 3
    • root
    • stem cell niche

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