Phase stretch transform for super-resolution localization microscopy

Tali Ilovitsh, Bahram Jalali, Mohammad H. Asghari, Zeev Zalevsky

Research output: Contribution to journalArticlepeer-review

Abstract

Super-resolution localization microscopy has revolutionized the observation of living structures at the cellular scale, by achieving a spatial resolution that is improved by more than an order of magnitude with compared to the diffraction limit. These methods localize single events from isolated sources in repeated cycles in order to achieve super-resolution. The requirement for sparse distribution of simultaneously activated sources in the field of view, dictates the acquisition of thousands of frames in order to construct the full super-resolution image. As a result, these methods have slow temporal resolution which is a major limitation when investigating live-cell dynamics. In this paper we present the use of phase stretch transform for high-density super-resolution localization microscopy. This is a nonlinear frequency dependent transform that emulates the propagation of light through a physical medium with specific warped diffractive property and applies a 2D phase function to the image in the frequency domain. By choosing properly the transform parameters and the phase kernel profile, the point spread function of each emitter can be sharpened and narrowed. This enables the localization of overlapping emitters, thus allows a higher density of activated emitters as well as shorter data collection acquisition rates. The method is validated by numerical simulations and by experimental data obtained using a microtubules sample.

Original languageEnglish
Article number#267193
Pages (from-to)4198-4209
Number of pages12
JournalBiomedical Optics Express
Volume7
Issue number10
DOIs
StatePublished - 1 Oct 2016
Externally publishedYes

Keywords

  • Fluorescence microscopy
  • Image processing
  • Image reconstruction techniques
  • Superresolution

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