TY - JOUR
T1 - Phagocytosis of Escherichia coli mediated by mannose resistant non-fimbrial haemagglutinin (NFA-1)
AU - Goldhar, Janina
AU - Yavzori, Miri
AU - Keisari, Yona
AU - Ofek, Itzhak
N1 - Funding Information:
This study was supported in part by a grant from the National Institute of Health, Al 23165 . The authors wish to thank Professor K . Jann and Dr B . Jann, Max-Planck Institut fur Immunbiologie, Freiburg, Germany, for their helpful discussions . We also thank Mrs Jehudith Grunberg, Blood Bank, Ichilov Hospital, Tel-Aviv for her useful advice in the assays of nonosponic phagocytosis of NFA-carrying E. coli .
PY - 1991/9
Y1 - 1991/9
N2 - We examined the interaction between a urinary isolate of E. coli carrying non-fimbrial MR adhesin (NFA-1) and human polymorphonuclear leukocytes (PMN). We found that the organisms attach to PMN and the attachment was dose dependent and saturable. It was inhibited by isolated NFA-1, but not by mannoside. Recombinant plasmid encoding NFA-1 adhesin conferred binding capacity on a non-adhesive strain. The attachment of the bacteria carrying NFA-1 to PMN was associated with oxidative burst and inhibited by isolated NFA-1. The data taken together suggest that NFA-1 mediates the attachment of the bacteria to PMN. The attachment is followed by ingestion and killing of the organisms, but at a slower rate than with bacteria bound via type 1 fimbriae.
AB - We examined the interaction between a urinary isolate of E. coli carrying non-fimbrial MR adhesin (NFA-1) and human polymorphonuclear leukocytes (PMN). We found that the organisms attach to PMN and the attachment was dose dependent and saturable. It was inhibited by isolated NFA-1, but not by mannoside. Recombinant plasmid encoding NFA-1 adhesin conferred binding capacity on a non-adhesive strain. The attachment of the bacteria carrying NFA-1 to PMN was associated with oxidative burst and inhibited by isolated NFA-1. The data taken together suggest that NFA-1 mediates the attachment of the bacteria to PMN. The attachment is followed by ingestion and killing of the organisms, but at a slower rate than with bacteria bound via type 1 fimbriae.
KW - E. coli haemagglutinin
KW - non-fimbrial adhesins
KW - phagocytosis
UR - http://www.scopus.com/inward/record.url?scp=0026215171&partnerID=8YFLogxK
U2 - 10.1016/0882-4010(91)90047-E
DO - 10.1016/0882-4010(91)90047-E
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AN - SCOPUS:0026215171
SN - 0882-4010
VL - 11
SP - 171
EP - 178
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
IS - 3
ER -