Phage display selection, analysis, and prediction of B Cell epitopes

Natalia Tarnovitski Freund*, David Enshell-Seijffers, Jonathan M. Gershoni

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

21 Scopus citations

Abstract

Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed "biopanning." The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided.

Original languageEnglish
Pages (from-to)9.8.1-9.8.30
JournalCurrent Protocols in Immunology
Issue numberSUPPL. 86
DOIs
StatePublished - 2009

Keywords

  • Epitope mapping
  • Mapitope
  • Mimotope
  • Pepitope
  • Peptide mimetics
  • Phage display
  • Random peptide libraries

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