Peritoneal ventilation with oxygen improves outcome after hemorrhagic shock in rats

Joseph Barr, Stephan Prueckner, Peter Safar*, Samuel A. Tisherman, Ann Radovsky, Jason Stezoski, Gideon Eshel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Objective: In experimental pulmonary consolidation with hypoxemia in rabbits, peritoneal ventilation (PV) with 100% oxygen (PV-O2) improved Pao2. We hypothesized that PV-O2 could improve outcome after hemorrhagic shock (HS) with normal lungs, by mitigating dysoxia of the abdominal viscera. Design: Randomized, controlled, laboratory animal study. Setting: University animal research facility. Subjective: Male Sprague-Dawley rats. Interventions: Thirty rats under light anesthesia (N2O/oxygen plus halothane) and spontaneous breathing underwent blood withdrawal of 3 mL/100) g over 15 mins. After volume-controlled HS phase 1 of 6O mins, resuscitation phase 2 of 60 mins included infusion of shed blood and, if necessary, additional lactated Ringer's solution intravenously to control normotension from to 120 mins. This was followed by observation phase 3 for 7 days. We randomized three groups of ten rats each: group I received PV-O2, starting at 15 mins of HS at a rate of 40 inflations/min, and a peritoneal "tidal volume" of 6 mL, until the end of phase 2. Group II received the same PV with room air (PV-Air). Control group III was treated without PV. Measurements and Main Results: During the second half of HS phase 1, mean arterial pressures were higher in the PV-O2 group I compared with the PV-Air group II and control group III (p < .05). All 30 rats survived the 120 mins of phases 1 and 2. Survival to 7 days was achieved by ten of ten rats in PV-O2 group I; by nine of ten in PV-Air group II; and by five of ten in control group III (p < .05 vs. group I; NS vs. group II). Survival times of <7 days were 5 days in the one death of group II and ranged between 6 hrs and 4 days in the five deaths of group III. In 7-day survivors, neurologic deficit scores (0% to 10% = normal, 100% = death) were normal, ranging between zero and 8%. Necropsies of rats that died during phase 3 showed multiple areas of necrosis of the gut, some with perforations. Necropsies in the five survivors to 7 days of group III showed marked macroscopic and microscopic changes (scattered areas of necrosis of stomach and intestine, adhesions, and pale areas in the liver). These changes were absent or less severe in the nine survivors of group II Viscera appeared normal in all ten rats of PV-O2 group I. Conclusions: Peritoneal ventilation with oxygen during and after severe hemorrhagic shock in rats seems to decrease morbidity and mortality by helping preserve viability of abdominal viscera.

Original languageEnglish
Pages (from-to)3896-3901
Number of pages6
JournalCritical Care Medicine
Volume28
Issue number12
DOIs
StatePublished - 2000

Keywords

  • Hemorrhagic shock
  • Multiple organ failure
  • Outcome model
  • Oxygen
  • Peritoneal oxygenation
  • Peritoneal ventilation
  • Rat model
  • Trauma resuscitation
  • Visceral ischemia

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