TY - JOUR
T1 - Peripheral-type benzodiazepine receptor ligands modulate human natural killer cell activity
AU - Bessler, Hanna
AU - Caspi, Beela
AU - Gavish, Moshe
AU - Rehavi, Moshe
AU - Hart, Jacob
AU - Weizman, Ronit
PY - 1997/5
Y1 - 1997/5
N2 - Following our earlier work, we evaluated the in vitro effect of ligands active at the peripheral-type benzodiazepine receptors on human natural killer cell activity. Peripheral blood mononuclear cells were incubated with benzodiazepine receptor ligands. After 4 h we observed a nonspecific inhibition of natural killer cell activity induced by both peripheral (Ro5-4864 and PK 11195) and central (clonazepam) benzodiazepine receptor ligands; after 24 h, the suppressive activity was specific to peripheral and mixed (diazepam) ligands, and the central-type ligand had no effect. This significant, specific suppression of NK cell activity was completely reversed by the addition of human recombinant interleukin-2 or human leukocyte interferon. Our research provides additional information on the immunomodulatory effects of peripheral-type benzodiazepine ligands. Further studies are needed to clarify the underlying mechanism of natural killer cell inhibition and to determine the clinical implications of these findings.
AB - Following our earlier work, we evaluated the in vitro effect of ligands active at the peripheral-type benzodiazepine receptors on human natural killer cell activity. Peripheral blood mononuclear cells were incubated with benzodiazepine receptor ligands. After 4 h we observed a nonspecific inhibition of natural killer cell activity induced by both peripheral (Ro5-4864 and PK 11195) and central (clonazepam) benzodiazepine receptor ligands; after 24 h, the suppressive activity was specific to peripheral and mixed (diazepam) ligands, and the central-type ligand had no effect. This significant, specific suppression of NK cell activity was completely reversed by the addition of human recombinant interleukin-2 or human leukocyte interferon. Our research provides additional information on the immunomodulatory effects of peripheral-type benzodiazepine ligands. Further studies are needed to clarify the underlying mechanism of natural killer cell inhibition and to determine the clinical implications of these findings.
KW - Cytotoxicity
KW - Interferon
KW - Interleukin-2
KW - Natural killer cells
KW - Peripheral-type benzodiazepine receptor
UR - http://www.scopus.com/inward/record.url?scp=0030727102&partnerID=8YFLogxK
U2 - 10.1016/S0192-0561(97)00013-1
DO - 10.1016/S0192-0561(97)00013-1
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AN - SCOPUS:0030727102
SN - 0192-0561
VL - 19
SP - 249
EP - 254
JO - International Journal of Immunopharmacology
JF - International Journal of Immunopharmacology
IS - 5
ER -