TY - JOUR
T1 - Period 2
T2 - A Regulator of Multiple Tissue-Specific Circadian Functions
AU - Ruggiero, Gennaro
AU - Ben-Moshe Livne, Zohar
AU - Wexler, Yair
AU - Geyer, Nathalie
AU - Vallone, Daniela
AU - Gothilf, Yoav
AU - Foulkes, Nicholas S.
N1 - Publisher Copyright:
© Copyright © 2021 Ruggiero, Ben-Moshe Livne, Wexler, Geyer, Vallone, Gothilf and Foulkes.
PY - 2021/9/3
Y1 - 2021/9/3
N2 - The zebrafish represents a powerful model for exploring how light regulates the circadian clock due to the direct light sensitivity of its peripheral clocks, a property that is retained even in organ cultures as well as zebrafish-derived cell lines. Light-inducible expression of the per2 clock gene has been predicted to play a vital function in relaying light information to the core circadian clock mechanism in many organisms, including zebrafish. To directly test the contribution of per2 to circadian clock function in zebrafish, we have generated a loss-of-function per2 gene mutation. Our results reveal a tissue-specific role for the per2 gene in maintaining rhythmic expression of circadian clock genes, as well as clock-controlled genes, and an impact on the rhythmic behavior of intact zebrafish larvae. Furthermore, we demonstrate that disruption of the per2 gene impacts on the circadian regulation of the cell cycle in vivo. Based on these results, we hypothesize that in addition to serving as a central element of the light input pathway to the circadian clock, per2 acts as circadian regulator of tissue-specific physiological functions in zebrafish.
AB - The zebrafish represents a powerful model for exploring how light regulates the circadian clock due to the direct light sensitivity of its peripheral clocks, a property that is retained even in organ cultures as well as zebrafish-derived cell lines. Light-inducible expression of the per2 clock gene has been predicted to play a vital function in relaying light information to the core circadian clock mechanism in many organisms, including zebrafish. To directly test the contribution of per2 to circadian clock function in zebrafish, we have generated a loss-of-function per2 gene mutation. Our results reveal a tissue-specific role for the per2 gene in maintaining rhythmic expression of circadian clock genes, as well as clock-controlled genes, and an impact on the rhythmic behavior of intact zebrafish larvae. Furthermore, we demonstrate that disruption of the per2 gene impacts on the circadian regulation of the cell cycle in vivo. Based on these results, we hypothesize that in addition to serving as a central element of the light input pathway to the circadian clock, per2 acts as circadian regulator of tissue-specific physiological functions in zebrafish.
KW - behavior
KW - cell cycle
KW - circadian clock
KW - metabolism
KW - period
KW - zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85115118004&partnerID=8YFLogxK
U2 - 10.3389/fnmol.2021.718387
DO - 10.3389/fnmol.2021.718387
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C2 - 34539343
AN - SCOPUS:85115118004
SN - 1662-5099
VL - 14
JO - Frontiers in Molecular Neuroscience
JF - Frontiers in Molecular Neuroscience
M1 - 718387
ER -