Performance of whole-cell electrochemical biosensor using integrated microbes/Si nano-forest structure

Nofar Hemed, Tal Yoetz-Kopelman, Annalisa Convertino, Yosi Shacham-Diamand

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A new structure, integrating microbial cells on Si nano-forest, combines whole cell biosensors with a functional nanostructured electrode is described in the current work. E.Coli microbes that expressed and displayed recombinant ZZ proteins were immobilized to a functionalized electrode using IgG antibodies. The presence of E.Coli cells was verified visually and electrochemically using scanning electron microscopy and impedance spectroscopy, respectively. Such structure produces unique electrochemical impedance spectrum that was affected by the analyte concentration. That signal, which is analyzed by both Nyquist diagram and the Bode plot, provide a proof of concept for the feasibility of such integrated whole cell biosensor. Moreover, the selectivity of the system was also confirmed using control cells displaying proteins different form ZZ.

Original languageEnglish
Title of host publicationChemical Sensors 12
Subtitle of host publicationChemical and Biological Sensors and Analytical Systems
EditorsM. Carter, Y. Shimizu, W. Y. Lee, T. Yasukawa, R. Mukundan, A. L. Simonian, L. A. Nagahara, O. Niwa, B. A. Chin
PublisherElectrochemical Society Inc.
Pages157-164
Number of pages8
Edition16
ISBN (Electronic)9781607687337, 9781607687672, 9781607687689, 9781607687696, 9781607687702, 9781607687719, 9781607687726, 9781607687733, 9781607687740, 9781607687757, 9781607687771, 9781607687788, 9781607687795, 9781607687801, 9781607687818, 9781607687825, 9781607687832, 9781607687849, 9781607687856, 9781607687863, 9781607687887, 9781607687894, 9781607687900, 9781607687917, 9781607687924, 9781607687931, 9781607687948, 9781607687955, 9781607687962, 9781607687979, 9781607687986, 9781607687993, 9781607688006, 9781607688013, 9781607688020, 9781607688037
DOIs
StatePublished - 2016
EventSymposium on Chemical Sensors 12: Chemical and Biological Sensors and Analytical Systems - PRiME 2016/230th ECS Meeting - Honolulu, United States
Duration: 2 Oct 20167 Oct 2016

Publication series

NameECS Transactions
Number16
Volume75
ISSN (Print)1938-6737
ISSN (Electronic)1938-5862

Conference

ConferenceSymposium on Chemical Sensors 12: Chemical and Biological Sensors and Analytical Systems - PRiME 2016/230th ECS Meeting
Country/TerritoryUnited States
CityHonolulu
Period2/10/167/10/16

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