Peptide self-assembly as a strategy for facile immobilization of redox enzymes on carbon electrodes

Itzhak Grinberg, Oren Ben-Zvi, Lihi Adler-Abramovich*, Iftach Yacoby*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Redox–enzyme-mediated electrochemical processes such as hydrogen production, nitrogen fixation, and CO2 reduction are at the forefront of the green chemistry revolution. To scale up, the inefficient two-dimensional (2D) immobilization of redox enzymes on working electrodes must be replaced by an efficient dense 3D system. Fabrication of 3D electrodes was demonstrated by embedding enzymes in polymer matrices. However, several requirements, such as simple immobilization, prolonged stability, and resistance to enzyme leakage, still need to be addressed. The study presented here aims to overcome these gaps by immobilizing enzymes in a supramolecular hydrogel formed by the self-assembly of the peptide hydrogelator fluorenylmethyloxycarbonyl-diphenylalanine. Harnessing the self-assembly process avoids the need for tedious and potentially harmful chemistry, allowing the rapid loading of enzymes on a 3D electrode under mild conditions. Using the [FeFe] hydrogenase enzyme, high enzyme loads, prolonged resistance against electrophoresis, and highly efficient hydrogen production are demonstrated. Further, this enzyme retention is shown to arise from its interaction with the peptide nanofibrils. Finally, this method is successfully used to retain other redox enzymes, paving the way for a variety of enzyme-mediated electrochemical applications.

Original languageEnglish
Article numbere411
JournalCarbon Energy
Issue number11
StatePublished - Nov 2023


FundersFunder number
Chaoul Center for Nanoscale Systems of Tel Aviv University
Israel Ministry of Energy 219‐11‐120222‐11‐065
Israel Science Foundation petroleum alternativesGA 2185/17
Ministry of Energy, Israel219-11-120


    • 3D electrode
    • H production
    • enzymes encapsulation
    • hydrogenase
    • peptide hydrogel


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