Pentraxin 3 and Shigella LPS and IpaB Antibodies Interplay to Defeat Shigellosis

Shiri Meron-Sudai, Arava Reizis, Sophy Goren, Anya Bialik, Amit Hochberg, Dani Cohen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Shigella causes moderate to severe diarrhea or dysentery after invading the colon mucosa. Long Pentraxin 3 (PTX3) is recognized as the humoral component of the innate immune response to bacterial pathogens. We examined the interplay between levels of PTX3 and levels of anti-Shigella lipopolysaccharide (LPS) and anti-Shigella type 3 secretion system protein-IpaB antibodies in children during acute shigellosis and after recovery. PTX3 concentrations in serum and stool extracts were determined by sandwich ELISA using commercial anti-PTX3 antibodies. Serum IgG, IgM, and IgA anti-S. sonnei LPS or anti-S. sonnei IpaB were measured using in house ELISA. Children with acute shigellosis (n = 60) had elevated PTX3 levels in serum and stools as compared with recovered subjects (9.6 ng/mL versus 4.7 ng/mL, p < 0.009 in serum and 16.3 ng/g versus 1.1 ng/g in stool, p = 0.011). Very low levels of PTX3 were detected in stools of healthy children (0.3 ng/g). Increased serum levels of PTX3 correlated with high fever accompanied by bloody or numerous diarrheal stools characteristic of more severe shigellosis while short pentraxin; C-Reactive Protein (CRP) did not show such a correlation. PTX3 decreased in convalescence while anti-Shigella antibodies increased, switching the response from innate to adaptive toward the eradication of the invasive organism. These data can inform the development of Shigella vaccines and treatment options.

Original languageEnglish
Article number4384
JournalJournal of Clinical Medicine
Issue number15
StatePublished - Aug 2022


  • Lipopolisaccharide (LPS)
  • Shigella
  • c-reactive protein (CRP)
  • invasion plasmid antigen B (IpaB)
  • pentraxin 3 (PTX3)


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