TY - JOUR
T1 - Penetration of moxifloxacin through crosslinked corneas
AU - Litvin, Gilad
AU - Ben Eliahu, Shmuel
AU - Rotenberg, Michael
AU - Marcovich, Arie L.
AU - Zadok, David
AU - Kleinmann, Guy
PY - 2014/7
Y1 - 2014/7
N2 - Purpose To determine the ability of moxifloxacin to penetrate the rabbit eye after corneal collagen crosslinking (CXL) with riboflavin and ultraviolet-A light irradiation. Setting Harlan Biotech Israel, Rehovot, Israel. Design Experimental study. Methods One eye of 10 New Zealand white rabbits had CXL treatment. One month after treatment and 1 hour before an aqueous humor sample was obtained, 1 drop of 5 mg/mL moxifloxacin (Vigamox) was applied to both eyes of each rabbit every 15 minutes for a total of 4 drops. The aqueous humor samples were sent for high-performance liquid chromatography for antibiotic-concentration analysis. The eyes were enucleated and sent for histology analysis. Results Moxifloxacin levels were obtained and analyzed for all 20 eyes. The mean level of moxifloxacin was 2.26 μg/mL ± 0.89 (SD) (range 1.09 to 4.20 μg/mL) in the treated eyes and 2.43 ± 1.17 μg/mL (range 0.89 to 4.72 μg/mL) in the untreated eyes. The difference between the groups was not statistically significant. Of the 10 eye pairs, lower moxifloxacin aqueous humor concentrations were found in 6 treated eyes and 4 untreated eyes. Conclusion Penetration of moxifloxacin into the anterior chamber of rabbits was not influenced by previous CXL treatment. Financial Disclosures No author has a financial or proprietary interest in any material or method mentioned.
AB - Purpose To determine the ability of moxifloxacin to penetrate the rabbit eye after corneal collagen crosslinking (CXL) with riboflavin and ultraviolet-A light irradiation. Setting Harlan Biotech Israel, Rehovot, Israel. Design Experimental study. Methods One eye of 10 New Zealand white rabbits had CXL treatment. One month after treatment and 1 hour before an aqueous humor sample was obtained, 1 drop of 5 mg/mL moxifloxacin (Vigamox) was applied to both eyes of each rabbit every 15 minutes for a total of 4 drops. The aqueous humor samples were sent for high-performance liquid chromatography for antibiotic-concentration analysis. The eyes were enucleated and sent for histology analysis. Results Moxifloxacin levels were obtained and analyzed for all 20 eyes. The mean level of moxifloxacin was 2.26 μg/mL ± 0.89 (SD) (range 1.09 to 4.20 μg/mL) in the treated eyes and 2.43 ± 1.17 μg/mL (range 0.89 to 4.72 μg/mL) in the untreated eyes. The difference between the groups was not statistically significant. Of the 10 eye pairs, lower moxifloxacin aqueous humor concentrations were found in 6 treated eyes and 4 untreated eyes. Conclusion Penetration of moxifloxacin into the anterior chamber of rabbits was not influenced by previous CXL treatment. Financial Disclosures No author has a financial or proprietary interest in any material or method mentioned.
UR - http://www.scopus.com/inward/record.url?scp=84903161550&partnerID=8YFLogxK
U2 - 10.1016/j.jcrs.2014.01.030
DO - 10.1016/j.jcrs.2014.01.030
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 24878254
AN - SCOPUS:84903161550
SN - 0886-3350
VL - 40
SP - 1177
EP - 1181
JO - Journal of Cataract and Refractive Surgery
JF - Journal of Cataract and Refractive Surgery
IS - 7
ER -