Abstract
One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 × 105 recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. Calmodulin expression was confirmed throughout maize embryogenesis at the mRNA, amplified cDNA and protein levels. Sequence analysis suggests a maize origin for both clones and negligible nucleotide changes linked to PCR. This library is the first described for early plant embryos and represents a breakthrough to isolate genes involved in embryo differentiation.
Original language | English |
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Pages (from-to) | 105-113 |
Number of pages | 9 |
Journal | Plant Molecular Biology |
Volume | 27 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1995 |
Externally published | Yes |
Keywords
- PCR
- Zea mays
- cDNA library
- calmodulin
- embryogenesis
- transition stage