PCR-generated cDNA library of transition-stage maize embryos: cloning and expression of calmodulin genes during early embryogenesis

Christian Breton*, Annie Chaboud, Elisabeth Matthys-Rochon, Elizabeth E.M. Bates, J. Mark Cock, Hillel Fromm, Christian Dumas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 × 105 recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. Calmodulin expression was confirmed throughout maize embryogenesis at the mRNA, amplified cDNA and protein levels. Sequence analysis suggests a maize origin for both clones and negligible nucleotide changes linked to PCR. This library is the first described for early plant embryos and represents a breakthrough to isolate genes involved in embryo differentiation.

Original languageEnglish
Pages (from-to)105-113
Number of pages9
JournalPlant Molecular Biology
Volume27
Issue number1
DOIs
StatePublished - Jan 1995
Externally publishedYes

Keywords

  • PCR
  • Zea mays
  • cDNA library
  • calmodulin
  • embryogenesis
  • transition stage

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