Patterns of protein kinase C isoenzyme expression in transitional cell carcinoma of bladder relation to degree of malignancy

L. Langzam, R. Koren, R. Gal, V. Kugel, A. Paz, A. Farkas, S. R. Sampson

Research output: Contribution to journalArticlepeer-review

Abstract

We determined the pattern of protein kinase C (PKC) isoform expression in human cell lines by Western blotting and immunofluorescent staining techniques. In addition, we examined PKC isoform expression in tissue samples of transitional cell carcinoma (TCC) of the bladder. PKC delta, PKC beta II, and PKC eta were found primarily in the RT4 cell line (low-grade tumor), and PKC zeta was expressed most strongly in the SUP cell line (invasive tumor). In tissue samples of urinary bladder cancer, PKC isoenzymes were expressed differentially as a function of tumor stage and grade; expression of PKC beta II and PKC delta was high in normal tissue and in lowgrade tumors and decreased with increasing stage and grade of TCC. The opposite pattern was seen with PKC zeta. The differences in expression of specific isoenzymes as related to levels of malignancy of the cell lines and tissue samples suggest that the PKC family has an important role in normal and neoplastic urothelium.

Original languageEnglish
Pages (from-to)377-385
Number of pages9
JournalAmerican Journal of Clinical Pathology
Volume116
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Cell culture
  • Isoenzymes
  • Malignancy
  • Protein kinase C
  • Urinary bladder

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