@article{0ab588448cda41319d9939ad74469f65,
title = "PASTICCINO1 (AtFKBP70) is a nuclear-localised immunophilin required during Arabidopsis thaliana embryogenesis",
abstract = "The PASTICCINO1 (PAS1) gene of Arabidopsis thaliana encodes a protein with homology to the FK506-binding protein (FKBP) class of immunophilins. To begin to understand more about the possible function of PAS1, we tested some properties of recombinant PAS1 protein and analysed the expression of the gene in Arabidopsis embryos and cell cultures and in tobacco cells. In pas1-1/+ heterozygote embryos the pas1-1 allele is expressed at very low levels in all cells, but it is misexpressed in the pas1-1 homozygote mutant at the same stage. Anti-PAS1 affinity-purified antibodies recognise a 70 kDa protein from dividing cell cultures of Arabidopsis. In indirect immunofluorescence, the same antibodies label the nuclei of dividing tobacco BY-2 cells. In a protease-coupled assay, recombinant PAS1 protein has low peptidylprolyl cis-trans isomerase (PPIase) activity, which is inhibited by the immunosuppressive drugs FK506 and rapamycin, but not by cyclosporin. PAS1 also binds calmodulin in vitro. This data suggests the importance of the correctly regulated production of functional PAS1 protein, a likely nuclear-localised FKBP, for the correct development of the plant embryo.",
keywords = "Arabidopsis, Calmodulin, Embryogenesis, FKBP, Nucleus, PPIase",
author = "Carol, {Rachel J.} and Adina Breiman and Noa Erel and Paola Vittorioso and Catherine Bellini",
note = "Funding Information: This work was funded by the European Community Framework IV LATIN (Lateral Initiation) project and by an Israeli Academy of Science grant to A. Breiman. We thank Yves Markowitz for help with spectrophotometry, Denis Falconet for the gift of the Arabidopsis and tobacco cells, and Regis Mache for permission to carry out many of these experiments in his laboratory. We thank Isaac Kurek, Jean-Denis Faure, Heather McKhann and Jacob Seeler for practical advice and useful discussions, Donald Richards for advice on the manuscript, and Pierre Carol for his support and encouragement for all aspects of this work.",
year = "2001",
doi = "10.1016/S0168-9452(01)00437-X",
language = "אנגלית",
volume = "161",
pages = "527--535",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "3",
}