TY - JOUR
T1 - Pasa
T2 - Proteomic analysis of serum antibodies web server
AU - Avram, Oren
AU - Kigel, Aya
AU - Vaisman-Mentesh, Anna
AU - Kligsberg, Sharon
AU - Rosenstein, Shai
AU - Dror, Yael
AU - Pupko, Tal
AU - Wine, Yariv
N1 - Publisher Copyright:
© 2021 Avram et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2021/1/25
Y1 - 2021/1/25
N2 - Motivation A comprehensive characterization of the humoral response towards a specific antigen requires quantification of the B-cell receptor repertoire (BCR-Seq) by next generation sequencing, as well as the analysis of serum antibodies against this antigen, using proteomics. The proteomic analysis is challenging since it necessitates the mapping of antigen-specific peptides to individual B-cell clones. Results The PASA web server provides a robust computational platform for the analysis and integration of data obtained from proteomics of serum antibodies. PASA maps peptides derived from antibodies raised against a specific antigen to corresponding antibody sequences. It then analyzes and integrates proteomics and BCR-Seq data, thus providing a comprehensive characterization of the humoral response. The PASA web server is freely available at https://pasa.tau.ac.il and open to all users without a login requirement.
AB - Motivation A comprehensive characterization of the humoral response towards a specific antigen requires quantification of the B-cell receptor repertoire (BCR-Seq) by next generation sequencing, as well as the analysis of serum antibodies against this antigen, using proteomics. The proteomic analysis is challenging since it necessitates the mapping of antigen-specific peptides to individual B-cell clones. Results The PASA web server provides a robust computational platform for the analysis and integration of data obtained from proteomics of serum antibodies. PASA maps peptides derived from antibodies raised against a specific antigen to corresponding antibody sequences. It then analyzes and integrates proteomics and BCR-Seq data, thus providing a comprehensive characterization of the humoral response. The PASA web server is freely available at https://pasa.tau.ac.il and open to all users without a login requirement.
UR - http://www.scopus.com/inward/record.url?scp=85100049757&partnerID=8YFLogxK
U2 - 10.1371/journal.pcbi.1008607
DO - 10.1371/journal.pcbi.1008607
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C2 - 33493161
AN - SCOPUS:85100049757
SN - 1553-734X
VL - 17
JO - PLoS Computational Biology
JF - PLoS Computational Biology
IS - 1
M1 - e1008607
ER -