Partial purification of scorpion hemocyanin messenger RNA

Ilana Avissar; Violet Daniel; Ezra Daniel

doi:10.1016/0305-0491(83)90334-6

Partial purification of scorpion hemocyanin messenger RNA

Ilana Avissar^*, Violet Daniel, Ezra Daniel

^*Corresponding author for this work

Biochemistry Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

1. 1. Antibodies raised to hemocyanin were used in the identification of in vitro synthesized hemocyanin among the products of cell-free translation of poly (A)⁺ mRNA from whole Leiurus quinquestriatus scorpions. 2. 2. Partial purification of hemocyanin mRNA was carried out by sucrose gradient fractionation. 3. 3. Evidence is presented that the message coding for hemocyanin is about 2000 nucleotides long. This value is consistent with the size of a constituent polypeptide chain of scorpion hemocyanin, about 75,000 daltons.

Original language	English
Pages (from-to)	327-330
Number of pages	4
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	75
Issue number	2
DOIs	https://doi.org/10.1016/0305-0491(83)90334-6
State	Published - 1983

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title = "Partial purification of scorpion hemocyanin messenger RNA",

abstract = "1. 1. Antibodies raised to hemocyanin were used in the identification of in vitro synthesized hemocyanin among the products of cell-free translation of poly (A)+ mRNA from whole Leiurus quinquestriatus scorpions. 2. 2. Partial purification of hemocyanin mRNA was carried out by sucrose gradient fractionation. 3. 3. Evidence is presented that the message coding for hemocyanin is about 2000 nucleotides long. This value is consistent with the size of a constituent polypeptide chain of scorpion hemocyanin, about 75,000 daltons.",

author = "Ilana Avissar and Violet Daniel and Ezra Daniel",

year = "1983",

doi = "10.1016/0305-0491(83)90334-6",

language = "אנגלית",

volume = "75",

pages = "327--330",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

issn = "1096-4959",

publisher = "Elsevier Inc.",

number = "2",

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T1 - Partial purification of scorpion hemocyanin messenger RNA

AU - Avissar, Ilana

AU - Daniel, Violet

AU - Daniel, Ezra

PY - 1983

Y1 - 1983

N2 - 1. 1. Antibodies raised to hemocyanin were used in the identification of in vitro synthesized hemocyanin among the products of cell-free translation of poly (A)+ mRNA from whole Leiurus quinquestriatus scorpions. 2. 2. Partial purification of hemocyanin mRNA was carried out by sucrose gradient fractionation. 3. 3. Evidence is presented that the message coding for hemocyanin is about 2000 nucleotides long. This value is consistent with the size of a constituent polypeptide chain of scorpion hemocyanin, about 75,000 daltons.

AB - 1. 1. Antibodies raised to hemocyanin were used in the identification of in vitro synthesized hemocyanin among the products of cell-free translation of poly (A)+ mRNA from whole Leiurus quinquestriatus scorpions. 2. 2. Partial purification of hemocyanin mRNA was carried out by sucrose gradient fractionation. 3. 3. Evidence is presented that the message coding for hemocyanin is about 2000 nucleotides long. This value is consistent with the size of a constituent polypeptide chain of scorpion hemocyanin, about 75,000 daltons.

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U2 - 10.1016/0305-0491(83)90334-6

DO - 10.1016/0305-0491(83)90334-6

M3 - מאמר

AN - SCOPUS:49049128170

VL - 75

SP - 327

EP - 330

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

SN - 1096-4959

IS - 2

ER -

Partial purification of scorpion hemocyanin messenger RNA

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