Partial characterization of three hemolymph proteins of Penaeus semisulcatus de haan (crustacea, decapoda, penaeidae) and their specific antibodies

Moshe Tom*, Ofer Shenker, Michael Ovadia

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

1. 1. LP1, a high density lipoprotein common to both sexes of the penaeid shrimp Penaeus semisulcatus was isolated as the upper yellow-brown layer resulting from ultracentrifugation of male hemolymph. The hemolymph solution was adjusted to a hydrated density of 1.2 g/ml. A similar HDL fraction was isolated from the hemolymph of vitellogenic females containing, in addition to the LP1, the female-specific lipoprotein vitellogenin (Vg). 2. 2. Hemocyanin (Hcy), the oxygen carrier of crustaceans was isolated as pellet, resulting from the same ultracentrifugation. Hcy was identified by its blue color, its copper content and its characteristic absorbance spectrum. It is the most abundant hemolymph protein, and is composed of two major subunits of 85 and 95 kD. 3. 3. Polyclonal antibodies were raised in rabbits against Hcy and LP1. The antisera were found to be specific and no crossreaction was observed between them and an anti-Vg/Vt serum prepared during earlier studies. All three antisera proved to be suitable research tools for the identification of these three physiologically important proteins (Vg, LP1, Hcy).

Original languageEnglish
Pages (from-to)811-816
Number of pages6
JournalComparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume104
Issue number4
DOIs
StatePublished - Apr 1993

Fingerprint

Dive into the research topics of 'Partial characterization of three hemolymph proteins of Penaeus semisulcatus de haan (crustacea, decapoda, penaeidae) and their specific antibodies'. Together they form a unique fingerprint.

Cite this