Parathyroid hormone effect on cell-to-cell communication in stromal and osteoblastic cells

R. Massas, D. Benayahu

Research output: Contribution to journalArticlepeer-review

Abstract

We characterized the formation and regulation of the gap junction in calvarial osteoblasts and in a series of subtypes from marrow stromal cells. The stromal cells included osteogenic, chondro-osteogenic, and endothelial cells. The cell coupling was measured by using fluorescence dye injected into single cells, and its migration to neighboring cells was measured. The functional coupling of cells was highly expressed by the osteoblastic cells. This process is mediated through fast changes in intracellular Ca+2 levels. Calcium ionophore (A 23187) demonstrated an uncoupling effect on the cells. In addition, the exposure of the cells to the parathyroid hormone increased the formation of the gap junction complex; the highest level was demonstrated in the osteoblastic cells.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalJournal of Cellular Biochemistry
Volume69
Issue number1
DOIs
StatePublished - 1 Apr 1998

Keywords

  • Cell communication
  • Osteoblasts
  • Stromal cells

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