Panoramic: A package for constructing eukaryotic pan-genomes

Lior Glick, Itay Mayrose*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The study of intraspecific genomic variation in eukaryotic species has been the focus of numerous genome resequencing projects in recent years. One emerging approach for the analysis of intraspecific diversity uses the concept of a pan-genome, which theoretically represents the full set of genomic sequences and coding genes from all individuals of a given species. This approach has many advantages over reference-based methods and has been successfully applied to study both prokaryotic and eukaryotic species. However, the process of pan-genome construction still presents considerable scientific and technical challenges, especially for eukaryotic species with large and complex genomes. Although general approaches for the construction of pan-genomes have been devised, currently available software tools implement only certain modules of the entire computational procedure. Therefore, each pan-genome project requires the development of tailored analysis pipelines, thus complicating and prolonging the process and impairing research reproducibility and comparison across studies. Here, we present Panoramic, a software package for the automatic construction of eukaryotic pan-genomes. Panoramic takes raw sequencing reads as input and applies two alternative approaches for pan-genome construction. Panoramic makes pan-genome construction a considerably easier task by providing simple user interface and efficient data processing algorithms. We demonstrate the use of Panoramic by constructing the pan-genome of the model plant species Arabidopsis thaliana from sequencing data of 20 diverse ecotypes.

Original languageEnglish
Pages (from-to)1393-1403
Number of pages11
JournalMolecular Ecology Resources
Issue number4
StatePublished - May 2021


FundersFunder number
United States - Israel Binational Agricultural Research and Development FundUS‐5089‐18


    • Arabidopsis thaliana
    • Pan-genome
    • assembly
    • gene content
    • genomics
    • presence/absence variation


    Dive into the research topics of 'Panoramic: A package for constructing eukaryotic pan-genomes'. Together they form a unique fingerprint.

    Cite this