Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain to Facilitate Peptide and Protein Synthesis

Suman Kumar Maity; Guy Mann; Muhammad Jbara; Shay Laps; Guy Kamnesky; Ashraf Brik

doi:10.1021/acs.orglett.6b01442

Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain to Facilitate Peptide and Protein Synthesis

Suman Kumar Maity, Guy Mann, Muhammad Jbara, Shay Laps, Guy Kamnesky, Ashraf Brik^*

^*Corresponding author for this work

Technion-Israel Institute of Technology

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Reversible attachment of solubilizing tags to hydrophobic peptides to facilitate their purification and ligation is an essential yet challenging task in chemical protein synthesis. The efficient palladium-assisted removal of the solubilizing tag linked to the Cys side chain is reported. The strategy was applied for the efficient preparation of histone protein H4 from two fragments via one-pot operation of ligation, removal of the solubilizing tag, and desulfurization.

Original language	English
Pages (from-to)	3026-3029
Number of pages	4
Journal	Organic Letters
Volume	18
Issue number	12
DOIs	https://doi.org/10.1021/acs.orglett.6b01442
State	Published - 17 Jun 2016
Externally published	Yes

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AU - Jbara, Muhammad

AU - Laps, Shay

AU - Kamnesky, Guy

AU - Brik, Ashraf

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Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain to Facilitate Peptide and Protein Synthesis

Abstract

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