P840-reaction centers from chlorobium tepidum - Qui none analysis and functional reconstitution into lipid vesicles

Nicole Frankenberg, Christine Hager-Braun, Ute Feiler, Markus Fuhrmann, Hans Rogl, Nikolaus Schneebauer, Nathan Nelson, Günter Hauska

Research output: Contribution to journalArticlepeer-review

Abstract

Membranes of Chlorobiuin tepidtim contain about 35, 45 and 2-10 molecules of menaquinone-7, chlorobium quinone (1′-oxo-menaquinone-7) and of the polar menaquinone (probably 1′-OH-menaquinone-7) per reaction center, respectively. None of these quinones was retained during the isolation of P840-reaction centers beyond the detection limit of about 0.2 quinones per reaction center, neither in the core complex nor in functionally intact reaction center preparations. The latter is shown to catalyze the formation of an electrochemical proton gradient in the presence of ascorbate and phenazinium methosulfate, when it is incorporated into lipid vesicles.

Original languageEnglish
Pages (from-to)14-19
Number of pages6
JournalPhotochemistry and Photobiology
Volume64
Issue number1
DOIs
StatePublished - 1996
Externally publishedYes

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