TY - JOUR
T1 - p63 protein is essential for the embryonic development of vibrissae and teeth
AU - Rufini, Alessandro
AU - Weil, Miguel
AU - McKeon, Frank
AU - Barlattani, Alberto
AU - Melino, Gerry
AU - Candi, Eleonora
N1 - Funding Information:
The authors are indebted to Mr. Alfredo Colangeli for the histology. The work was supported by grants from Telethon to E.C., AIRC, Telethon, EU, FIRB, MIUR, MinSan, Telethon to G.M., and Medical Research Council to G.M. M. Weil was supported by EMBO short-term fellowship.
PY - 2006/2/17
Y1 - 2006/2/17
N2 - Development of skin appendages strongly depends on epithelial-mesenchymal interactions. One of the genes involved in this process is p63, a member of the p53 family of transcription factors, essential for ectodermal development, as elucidated by the phenotype of p63 knock-out mice. Surprisingly, no information on p63 expression in tooth and hair is yet available. Here, we show p63 expression during teeth and vibrissae morphogenesis in mouse embryos and we also show a correlation with the expression patterns of the epithelial marker keratin 5 and the proliferation marker Ki67. Our results show that p63 colocalizes with both K5 and Ki67 in the epithelium of developing vibrissae, while in teeth p63 is expressed, together with K5, in the undifferentiated ectoderm (enamel organ), and in ameloblasts, a subpopulation of differentiated ectodermal cells. Moreover, p63 expression in tooth seems not to be fully colocalized with nuclear Ki67 expression.
AB - Development of skin appendages strongly depends on epithelial-mesenchymal interactions. One of the genes involved in this process is p63, a member of the p53 family of transcription factors, essential for ectodermal development, as elucidated by the phenotype of p63 knock-out mice. Surprisingly, no information on p63 expression in tooth and hair is yet available. Here, we show p63 expression during teeth and vibrissae morphogenesis in mouse embryos and we also show a correlation with the expression patterns of the epithelial marker keratin 5 and the proliferation marker Ki67. Our results show that p63 colocalizes with both K5 and Ki67 in the epithelium of developing vibrissae, while in teeth p63 is expressed, together with K5, in the undifferentiated ectoderm (enamel organ), and in ameloblasts, a subpopulation of differentiated ectodermal cells. Moreover, p63 expression in tooth seems not to be fully colocalized with nuclear Ki67 expression.
KW - Hair
KW - Keratin
KW - Skin appendage
KW - Tooth
KW - p63
UR - http://www.scopus.com/inward/record.url?scp=30144440831&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.12.065
DO - 10.1016/j.bbrc.2005.12.065
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AN - SCOPUS:30144440831
SN - 0006-291X
VL - 340
SP - 737
EP - 741
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -