P53 Gene Mutation in a T-Acute Lymphoblastic Leukemia Cell Line (Loucy) with t(16:20) and 5q-Chromosomal Aberrations

Miron Prokocimer, Shoshana Peller, Hannah Ben-Bassat, Naomi Goldfinger, Varda Rotter

Research output: Contribution to journalArticlepeer-review

Abstract

A human T-acute lymphoblastic leukemia (ALL) cell line (Loucy), derived from cells from a patient with resistant ALL with a t(16:20) and 5q-chromosomal aberrations was evaluated for p53 gene alterations and expression. Western blot analysis of p53 showed elevated levels of the protein. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and direct sequencing identified a point mutation at codon 272 (GTG → ATG) of the p53 gene. Possible molecular mechanisms underlying these alterations and their role in the establishment of this cell line and in leukemogenesis in general are discussed.

Original languageEnglish
Pages (from-to)607-611
Number of pages5
JournalLeukemia and Lymphoma
Volume29
Issue number42130
DOIs
StatePublished - 1 Jan 1998
Externally publishedYes

Keywords

  • ALL
  • Tumor suppressor
  • codon 272
  • mutation
  • p53

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