Oxidative crosslinking of LDL protein induced by hemin: Involvement of tyrosines

Y. I. Miller, N. Shaklai*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


This study investigated free hemin induced modifications in low density lipoprotein (LDL). By use of fluorescent probes hemin was found to associate with LDL thereby inducing peroxidation of both lipids and protein. Upon LDL peroxidation, covalent crosslinking of apolipoprotein B (Apo B) occurred as judged by SDS-PAGE. Concomitantly, a multifluorophore emission developed, which included contribution of bityrosines. The simultaneous formation of protein aggregates and bityrosines was interpreted as the involvement of intermolecular bityrosines in the hemin induced crosslinking of Apo B. Since LDL protein aggregation relates to conversion of macrophages into foam cells, hemin should be considered as an endogenous trigger of atherosclerosis.

Original languageEnglish
Pages (from-to)1121-1129
Number of pages9
JournalBiochemistry and Molecular Biology International
Issue number6
StatePublished - 1994


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