Overexpression of human p21waf1/cip1 arrests the growth of chicken embryo fibroblasts transformed by individual oncogenes

Iris Givol; David Givol; Shen Rulong; James Resau; Ilan Tsarfaty; Stephen H. Hughes

Overexpression of human p21^waf1/cip1 arrests the growth of chicken embryo fibroblasts transformed by individual oncogenes

Iris Givol, David Givol, Shen Rulong, James Resau, Ilan Tsarfaty, Stephen H. Hughes^*

^*Corresponding author for this work

Clinical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

In normal cells, cell growth and division is controlled by the interplay between proto-oncogenes and tumor suppressor genes. Cancer cells usually have both activated an oncogene and have lost a functional tumor suppressor gene. High level expression of a tumor suppressor, p53, can block the growth of cancer cells. waf1/cip1 is transactivated by the tumor suppressor p53 and the p21^waf1/cip1protein is itself a suppressor of cell growth. To test the effect of growth suppression genes on the growth of cells transformed by individual oncogenes, we have used replication-competent retroviral vectors to induce high level expression of p53 and p21^waf1/cip1. Overexpression of p21^waf1/cip1 arrests the growth of chicken embryo fibroblasts (CEF) transformed by v- Src, tf-Ras, c-Mos and c-Myc. These data suggest that p21^waf1/cip1 might be a useful tool in gene therapy for human cancer.

Original language	English
Pages (from-to)	2608-2618
Number of pages	11
Journal	Oncogene
Volume	11
Issue number	12
State	Published - 21 Dec 1995

Keywords

Cell cycle
Growth suppressor
Retrovirus
p53
waf1/cip1

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title = "Overexpression of human p21waf1/cip1 arrests the growth of chicken embryo fibroblasts transformed by individual oncogenes",

abstract = "In normal cells, cell growth and division is controlled by the interplay between proto-oncogenes and tumor suppressor genes. Cancer cells usually have both activated an oncogene and have lost a functional tumor suppressor gene. High level expression of a tumor suppressor, p53, can block the growth of cancer cells. waf1/cip1 is transactivated by the tumor suppressor p53 and the p21waf1/cip1protein is itself a suppressor of cell growth. To test the effect of growth suppression genes on the growth of cells transformed by individual oncogenes, we have used replication-competent retroviral vectors to induce high level expression of p53 and p21waf1/cip1. Overexpression of p21waf1/cip1 arrests the growth of chicken embryo fibroblasts (CEF) transformed by v- Src, tf-Ras, c-Mos and c-Myc. These data suggest that p21waf1/cip1 might be a useful tool in gene therapy for human cancer.",

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author = "Iris Givol and David Givol and Shen Rulong and James Resau and Ilan Tsarfaty and Hughes, {Stephen H.}",

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T1 - Overexpression of human p21waf1/cip1 arrests the growth of chicken embryo fibroblasts transformed by individual oncogenes

AU - Givol, Iris

AU - Givol, David

AU - Rulong, Shen

AU - Resau, James

AU - Tsarfaty, Ilan

AU - Hughes, Stephen H.

PY - 1995/12/21

Y1 - 1995/12/21

N2 - In normal cells, cell growth and division is controlled by the interplay between proto-oncogenes and tumor suppressor genes. Cancer cells usually have both activated an oncogene and have lost a functional tumor suppressor gene. High level expression of a tumor suppressor, p53, can block the growth of cancer cells. waf1/cip1 is transactivated by the tumor suppressor p53 and the p21waf1/cip1protein is itself a suppressor of cell growth. To test the effect of growth suppression genes on the growth of cells transformed by individual oncogenes, we have used replication-competent retroviral vectors to induce high level expression of p53 and p21waf1/cip1. Overexpression of p21waf1/cip1 arrests the growth of chicken embryo fibroblasts (CEF) transformed by v- Src, tf-Ras, c-Mos and c-Myc. These data suggest that p21waf1/cip1 might be a useful tool in gene therapy for human cancer.

AB - In normal cells, cell growth and division is controlled by the interplay between proto-oncogenes and tumor suppressor genes. Cancer cells usually have both activated an oncogene and have lost a functional tumor suppressor gene. High level expression of a tumor suppressor, p53, can block the growth of cancer cells. waf1/cip1 is transactivated by the tumor suppressor p53 and the p21waf1/cip1protein is itself a suppressor of cell growth. To test the effect of growth suppression genes on the growth of cells transformed by individual oncogenes, we have used replication-competent retroviral vectors to induce high level expression of p53 and p21waf1/cip1. Overexpression of p21waf1/cip1 arrests the growth of chicken embryo fibroblasts (CEF) transformed by v- Src, tf-Ras, c-Mos and c-Myc. These data suggest that p21waf1/cip1 might be a useful tool in gene therapy for human cancer.

KW - Cell cycle

KW - Growth suppressor

KW - Retrovirus

KW - p53

KW - waf1/cip1

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SN - 0950-9232

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JO - Oncogene

JF - Oncogene

IS - 12

ER -

Overexpression of human p21^waf1/cip1 arrests the growth of chicken embryo fibroblasts transformed by individual oncogenes

Abstract

Keywords

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