Overexpression of human p21waf1/cip1 arrests the growth of chicken embryo fibroblasts transformed by individual oncogenes

Iris Givol, David Givol, Shen Rulong, James Resau, Ilan Tsarfaty, Stephen H. Hughes

Research output: Contribution to journalArticlepeer-review

Abstract

In normal cells, cell growth and division is controlled by the interplay between proto-oncogenes and tumor suppressor genes. Cancer cells usually have both activated an oncogene and have lost a functional tumor suppressor gene. High level expression of a tumor suppressor, p53, can block the growth of cancer cells. waf1/cip1 is transactivated by the tumor suppressor p53 and the p21waf1/cip1protein is itself a suppressor of cell growth. To test the effect of growth suppression genes on the growth of cells transformed by individual oncogenes, we have used replication-competent retroviral vectors to induce high level expression of p53 and p21waf1/cip1. Overexpression of p21waf1/cip1 arrests the growth of chicken embryo fibroblasts (CEF) transformed by v- Src, tf-Ras, c-Mos and c-Myc. These data suggest that p21waf1/cip1 might be a useful tool in gene therapy for human cancer.

Original languageEnglish
Pages (from-to)2608-2618
Number of pages11
JournalOncogene
Volume11
Issue number12
StatePublished - 21 Dec 1995

Keywords

  • Cell cycle
  • Growth suppressor
  • Retrovirus
  • p53
  • waf1/cip1

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