TY - JOUR
T1 - Over-expression of the functional interleukin-11α receptor in the development of B-cell chronic lymphocytic leukemia
AU - Tsimanis, A.
AU - Shvidel, L.
AU - Klepfish, A.
AU - Shtalrid, M.
AU - Kalinkovich, A.
AU - Berrebi, A.
N1 - Funding Information:
We thank Prof. I. Lefkovits (Bazel Institute for Immunology), Prof. S. Einhorn (Karolinska Institutet) for many helpful comments on the work and critically reading the manuscript and for the excellent assistance given by Mrs. J. Rosensaft (Kaplan Medical Center, Rehovot). This work was supported in part by Ministry of Absorption of Israel.
PY - 2001
Y1 - 2001
N2 - Several cytokines have been found to play a role in the pathogenesis of B-CLL. In the present study using reverse-transcriptase polymerase chain reaction (RT-PCR), FACS analysis and immunofluorescence we have shown the constitutive expression of IL-11 and IL-11Rα in B-chronic lymphocytic leukemia (B-CLL). The expression level of IL-11Rα in B-CLL cells is much higher than in PBL of normal donors. Recombinant human IL-11 (rhIL-11) activates B-CLL cells, leading to morphologic alterations of the cells and increase in cell number and size. Short-term cultivation in the presence of rhIL-11 did not lead to quantitative changes in the ratio of the living vs apoptotic and dead cells. However, in contrast to rhIL-6, pretreatment with rhIL-11, did not cause B-CLL cells to be resistant to the action of dexamethasone. These data suggest an essential role for the IL-11/IL-11Rα system in the pathogenesis of the malignant B-CLL cells.
AB - Several cytokines have been found to play a role in the pathogenesis of B-CLL. In the present study using reverse-transcriptase polymerase chain reaction (RT-PCR), FACS analysis and immunofluorescence we have shown the constitutive expression of IL-11 and IL-11Rα in B-chronic lymphocytic leukemia (B-CLL). The expression level of IL-11Rα in B-CLL cells is much higher than in PBL of normal donors. Recombinant human IL-11 (rhIL-11) activates B-CLL cells, leading to morphologic alterations of the cells and increase in cell number and size. Short-term cultivation in the presence of rhIL-11 did not lead to quantitative changes in the ratio of the living vs apoptotic and dead cells. However, in contrast to rhIL-6, pretreatment with rhIL-11, did not cause B-CLL cells to be resistant to the action of dexamethasone. These data suggest an essential role for the IL-11/IL-11Rα system in the pathogenesis of the malignant B-CLL cells.
KW - B-chronic lymphocytic leukemia
KW - Expression
KW - Interleukin-11
KW - Interleukin-11 α receptor
UR - http://www.scopus.com/inward/record.url?scp=0034920163&partnerID=8YFLogxK
U2 - 10.3109/10428190109097691
DO - 10.3109/10428190109097691
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 11699207
AN - SCOPUS:0034920163
SN - 1042-8194
VL - 42
SP - 195
EP - 205
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
IS - 1-2
ER -
