Over-expression of the functional interleukin-11α receptor in the development of B-cell chronic lymphocytic leukemia

A. Tsimanis, L. Shvidel, A. Klepfish, M. Shtalrid, A. Kalinkovich, A. Berrebi

Research output: Contribution to journalArticlepeer-review

Abstract

Several cytokines have been found to play a role in the pathogenesis of B-CLL. In the present study using reverse-transcriptase polymerase chain reaction (RT-PCR), FACS analysis and immunofluorescence we have shown the constitutive expression of IL-11 and IL-11Rα in B-chronic lymphocytic leukemia (B-CLL). The expression level of IL-11Rα in B-CLL cells is much higher than in PBL of normal donors. Recombinant human IL-11 (rhIL-11) activates B-CLL cells, leading to morphologic alterations of the cells and increase in cell number and size. Short-term cultivation in the presence of rhIL-11 did not lead to quantitative changes in the ratio of the living vs apoptotic and dead cells. However, in contrast to rhIL-6, pretreatment with rhIL-11, did not cause B-CLL cells to be resistant to the action of dexamethasone. These data suggest an essential role for the IL-11/IL-11Rα system in the pathogenesis of the malignant B-CLL cells.

Original languageEnglish
Pages (from-to)195-205
Number of pages11
JournalLeukemia and Lymphoma
Volume42
Issue number1-2
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • B-chronic lymphocytic leukemia
  • Expression
  • Interleukin-11
  • Interleukin-11 α receptor

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