Over-expression of highly conserved mitochondrial 70-kDa heat-shock protein in the sea anemone Anemonia viridis

Omer Choresh*, Abdussalam Azem, Yossi Loya

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Organisms react to cellular stress by inducing the synthesis of heat-shock proteins (Hsp). One such protein is the mitochondrial 70-kDa Hsp (mHsp70). The expression of mHsp70 in organisms that undergo stress in their natural habitat is unknown. We used a biochemical approach enabling to identify an mHsp70 from the sea anemone Anemonia viridis, which is abundant in highly fluctuating marine habitats. Antibodies raised specifically against yeast mHsp70 recognized a 70-kDa protein from A. viridis. We found that A. viridis mHsp70 is constitutively expressed at 22-23 °C, but over-expressed upon exposure to heat shock (31 °C) or to temperature fluctuations, suggesting that mHsp70 plays a significant role in adaptation of sessile marine invertebrates to highly fluctuating environmental conditions. Using an affinity column we were able to obtain a partially purified fraction of this protein. Partial amino acid sequences proved that the purified Hsp70 functions in the mitochondria.

Original languageEnglish
Pages (from-to)367-373
Number of pages7
JournalJournal of Thermal Biology
Volume32
Issue number7-8
DOIs
StatePublished - Oct 2007

Funding

FundersFunder number
Porter School for Environmental Studies
German-Israeli Foundation for Scientific Research and Development753/2002

    Keywords

    • Anemonia viridis
    • Environmental stress
    • Mitochondrial Hsp70

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