TY - JOUR
T1 - Ovarian angiotensin-converting enzyme activity in humans
T2 - Relationship to estradiol, age, and uterine pathology
AU - Erman, Arie
AU - Chen-Gal, Batia
AU - Van Dijk, David J.
AU - Sulkes, Jaqueline
AU - Kaplan, Boris
AU - Boner, Geoffrey
AU - Neri, Alexander
PY - 1996
Y1 - 1996
N2 - The present study was designed to measure angiotensin-converting enzyme (ACE) activity in the human ovary and in serum and to relate this activity to age, serum estradiol levels, and uterine and endometrial pathology. ACE activity was determined in 56 females by a radiometric assay using [3H]hippuryl-glycyl-glycine as substrate. Ovarian ACE activity, but not serum ACE, was found to increase with age (P < 0.01) and was significantly greater in postmenopausal subjects (n = 31; 1.35 ± 0.05 nmol/mg · min) than in subjects with active ovaries (n = 21; 0.65 ± 0.2 nmol/mg · min; P = 0.0033). Ovarian ACE activities in fertile women in the preovulatory phase (n = 14) and the postovulatory phase (n = 7) were not statistically different (0.66 ± 0.23 and 0.60 ± 0.17 nmol/mg · min, respectively). Serum ACE activities were similar in females with active and nonactive ovaries (87.6 ± 5.0 vs. 81.7 ± 5.3 nmol/mL · min, respectively). Serum estradiol levels in fertile women were significantly higher than those in postmenopausal women (P = 0.0023). Serum estradiol levels were negatively correlated with age (r = - 0.46; P = 0.0041) and were not correlated with either serum ACE activity (r = 0.080; P = NS) or ovarian ACE activity. In summary, human ovarian ACE activity, but not serum ACE, is positively correlated with age. Serum estradiol levels decrease with age, but are not correlated with either ovarian or serum ACE activity. Endogenous serum estradiol levels had no apparent effect on ovarian or serum ACE activity. The presence of uterine pathology affects ovarian ACE activity. The cause of the increased ovarian ACE activity is not clear, but may be related to the aging process.
AB - The present study was designed to measure angiotensin-converting enzyme (ACE) activity in the human ovary and in serum and to relate this activity to age, serum estradiol levels, and uterine and endometrial pathology. ACE activity was determined in 56 females by a radiometric assay using [3H]hippuryl-glycyl-glycine as substrate. Ovarian ACE activity, but not serum ACE, was found to increase with age (P < 0.01) and was significantly greater in postmenopausal subjects (n = 31; 1.35 ± 0.05 nmol/mg · min) than in subjects with active ovaries (n = 21; 0.65 ± 0.2 nmol/mg · min; P = 0.0033). Ovarian ACE activities in fertile women in the preovulatory phase (n = 14) and the postovulatory phase (n = 7) were not statistically different (0.66 ± 0.23 and 0.60 ± 0.17 nmol/mg · min, respectively). Serum ACE activities were similar in females with active and nonactive ovaries (87.6 ± 5.0 vs. 81.7 ± 5.3 nmol/mL · min, respectively). Serum estradiol levels in fertile women were significantly higher than those in postmenopausal women (P = 0.0023). Serum estradiol levels were negatively correlated with age (r = - 0.46; P = 0.0041) and were not correlated with either serum ACE activity (r = 0.080; P = NS) or ovarian ACE activity. In summary, human ovarian ACE activity, but not serum ACE, is positively correlated with age. Serum estradiol levels decrease with age, but are not correlated with either ovarian or serum ACE activity. Endogenous serum estradiol levels had no apparent effect on ovarian or serum ACE activity. The presence of uterine pathology affects ovarian ACE activity. The cause of the increased ovarian ACE activity is not clear, but may be related to the aging process.
UR - http://www.scopus.com/inward/record.url?scp=0029924183&partnerID=8YFLogxK
U2 - 10.1210/jc.81.3.1104
DO - 10.1210/jc.81.3.1104
M3 - מאמר
AN - SCOPUS:0029924183
VL - 81
SP - 1104
EP - 1107
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 3
ER -