Ornithine-δ-transaminase from the liver fluke Fasciola hepatica and the blood fluke Schistosoma mansoni: A comparative study

M. Goldberg, E. Flescher, D. Gold, J. Lengy

Research output: Contribution to journalArticlepeer-review

Abstract

1. 1. The biochemical properties of ornithine-δ-transaminase (OTA) from Schistosoma mansoni and Fasciola hepatica were compared. 2. 2. The OTA from both flukes could be solubilized to different degrees by various detergent mixtures. 3. 3. The pH optimum for the enzymatic activity was 8-8.5. 4. 4. The Km values of OTA from F. hepatica were 3.29 and 1.81 mM for ornithine and α-ketoglutarate, respectively, and of OTA from S. mansoni-1.53 and 2.07 mM. 5. 5. The enzyme showed the highest affinity for α-ketoglutarate from among seven keto acids evaluated. 6. 6. OTA was inhibited by known pyridoxal phosphate inhibitors as well as by several structural analogues of its two substrates.

Original languageEnglish
Pages (from-to)605-613
Number of pages9
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume65
Issue number4
DOIs
StatePublished - 1980

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