TY - JOUR
T1 - Organic fluorescent reagents. XIV. Novel fluorogenic substrates for microdetermination of chymotrypsin and aminopeptidase
T2 - Bimane fluorescence appears after hydrolysis
AU - Sato, Eisuke
AU - Sakashita, Mari
AU - Kanaoka, Yuichi
AU - Kosower, Edward M.
PY - 1988/9
Y1 - 1988/9
N2 - The fluorescence of 9,10-dioxa-syn-3,4,6,7-tetramethylbimane (bimane) was found to be quenched in the presence of tryptophan or tyrosine. Based on this observation, the bimane system was utilized as a fluorophore within proteolytic enzyme substrates. Bimane peptides containing tryptophan (1a-1f) were prepared and shown to be potent fluorogenic substrates for the assay of chymotrypsin and aminopeptidase.
AB - The fluorescence of 9,10-dioxa-syn-3,4,6,7-tetramethylbimane (bimane) was found to be quenched in the presence of tryptophan or tyrosine. Based on this observation, the bimane system was utilized as a fluorophore within proteolytic enzyme substrates. Bimane peptides containing tryptophan (1a-1f) were prepared and shown to be potent fluorogenic substrates for the assay of chymotrypsin and aminopeptidase.
UR - http://www.scopus.com/inward/record.url?scp=0023742672&partnerID=8YFLogxK
U2 - 10.1016/0045-2068(88)90017-X
DO - 10.1016/0045-2068(88)90017-X
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AN - SCOPUS:0023742672
SN - 0045-2068
VL - 16
SP - 298
EP - 306
JO - Bioorganic Chemistry
JF - Bioorganic Chemistry
IS - 3
ER -