TY - JOUR
T1 - Organ-injury-induced reactivation of hemangioblastic precursor cells
AU - Dekel, B.
AU - Metsuyanim, S.
AU - Garcia, A. M.
AU - Quintero, C.
AU - Sanchez, M. J.
AU - Izraeli, S.
N1 - Funding Information:
This study was partially supported by grants from the Israel Scientific Foundation Physician-Scientist Grant Award, Sheba Career Development Award and Moriss Kahn Career Development Award (BD); The Israel Cancer Research Foundation and the Recannati foundation (SI); The Spanish Ministry of Education and Science Grant SAF07241; Junta de Andalucia grant PAI-CVI 295, fellowship CONACYT179065 to AMG and fellowship I3P-CSIC to CQ.
PY - 2008/1
Y1 - 2008/1
N2 - Early in mammalian development, the stem cell leukemia (SCL/TAL1) gene and its distinct 3′ enhancer (SCL 3′En) specify bipotential progenitor cells that give rise to blood and endothelium, thus termed hemangioblasts. We have previously detected a minor population of SCL (+) cells in the postnatal kidney. Here, we demonstrate that cells expressing the SCL 3′En in the adult kidney are comprised of CD45+CD31- hematopoietic cells, CD45-CD31+ endothelial cells and CD45-CD31- interstitial cells. Creation of bone marrow chimeras of SCL 3′En transgenic mice into wild-type hosts shows that all three types of SCL 3′En-expressing cells in the adult kidney can originate from the bone marrow. Ischemia/reperfusion injury to the adult kidney of SCL 3′En transgenic mice results in the intrarenal elevation of SCL and FLK1 mRNA levels and of cells expressing hem-endothelial progenitor markers (CD45, CD34, c-Kit and FLK1). Furthermore, analysis of SCL 3′En in the ischemic kidneys reveals an increase in the abundance of SCL 3′En-expressing cells, predominantly within the CD45 (+) hematopoietic fraction and to a lesser extent in the CD45 (-) fraction. Our results suggest organ-injury-induced reactivation of bone marrow-derived hemangioblasts and possible local angioblastic progenitors expressing SCL and SCL 3′.
AB - Early in mammalian development, the stem cell leukemia (SCL/TAL1) gene and its distinct 3′ enhancer (SCL 3′En) specify bipotential progenitor cells that give rise to blood and endothelium, thus termed hemangioblasts. We have previously detected a minor population of SCL (+) cells in the postnatal kidney. Here, we demonstrate that cells expressing the SCL 3′En in the adult kidney are comprised of CD45+CD31- hematopoietic cells, CD45-CD31+ endothelial cells and CD45-CD31- interstitial cells. Creation of bone marrow chimeras of SCL 3′En transgenic mice into wild-type hosts shows that all three types of SCL 3′En-expressing cells in the adult kidney can originate from the bone marrow. Ischemia/reperfusion injury to the adult kidney of SCL 3′En transgenic mice results in the intrarenal elevation of SCL and FLK1 mRNA levels and of cells expressing hem-endothelial progenitor markers (CD45, CD34, c-Kit and FLK1). Furthermore, analysis of SCL 3′En in the ischemic kidneys reveals an increase in the abundance of SCL 3′En-expressing cells, predominantly within the CD45 (+) hematopoietic fraction and to a lesser extent in the CD45 (-) fraction. Our results suggest organ-injury-induced reactivation of bone marrow-derived hemangioblasts and possible local angioblastic progenitors expressing SCL and SCL 3′.
UR - http://www.scopus.com/inward/record.url?scp=38349049167&partnerID=8YFLogxK
U2 - 10.1038/sj.leu.2404941
DO - 10.1038/sj.leu.2404941
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C2 - 17898790
AN - SCOPUS:38349049167
VL - 22
SP - 103
EP - 113
JO - Leukemia
JF - Leukemia
SN - 0887-6924
IS - 1
ER -
