Organ-injury-induced reactivation of hemangioblastic precursor cells

B. Dekel, S. Metsuyanim, A. M. Garcia, C. Quintero, M. J. Sanchez, S. Izraeli

Research output: Contribution to journalArticlepeer-review

Abstract

Early in mammalian development, the stem cell leukemia (SCL/TAL1) gene and its distinct 3′ enhancer (SCL 3′En) specify bipotential progenitor cells that give rise to blood and endothelium, thus termed hemangioblasts. We have previously detected a minor population of SCL (+) cells in the postnatal kidney. Here, we demonstrate that cells expressing the SCL 3′En in the adult kidney are comprised of CD45+CD31- hematopoietic cells, CD45-CD31+ endothelial cells and CD45-CD31- interstitial cells. Creation of bone marrow chimeras of SCL 3′En transgenic mice into wild-type hosts shows that all three types of SCL 3′En-expressing cells in the adult kidney can originate from the bone marrow. Ischemia/reperfusion injury to the adult kidney of SCL 3′En transgenic mice results in the intrarenal elevation of SCL and FLK1 mRNA levels and of cells expressing hem-endothelial progenitor markers (CD45, CD34, c-Kit and FLK1). Furthermore, analysis of SCL 3′En in the ischemic kidneys reveals an increase in the abundance of SCL 3′En-expressing cells, predominantly within the CD45 (+) hematopoietic fraction and to a lesser extent in the CD45 (-) fraction. Our results suggest organ-injury-induced reactivation of bone marrow-derived hemangioblasts and possible local angioblastic progenitors expressing SCL and SCL 3′.

Original languageEnglish
Pages (from-to)103-113
Number of pages11
JournalLeukemia
Volume22
Issue number1
DOIs
StatePublished - Jan 2008

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